Anti-Cytokeratin 14 Antibody

Cat: 100129-R101
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Anti-Cytokeratin 14 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Cytokeratin 14 Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human Cytokeratin 14
Immunogen
A synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14.
Preparation
This antibody was obtained from a rabbit immunized with purified, a synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14.
Source
Monoclonal Rabbit IgG Clone #101
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cytokeratin 14 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:10000-1:20000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cytokeratin 14 Antibody (Rabbit Monoclonal antibody) Images
Human KRT14 Immunohistochemistry(IHC) 4411
Immunochemical staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Human KRT14 Immunohistochemistry(IHC) 4413
Immunochemical staining of human CK14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Human KRT14 Immunoprecipitation(IP) 6112

KRT14 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

2 µL anti-KRT14 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 52 kDa

Observed band size: 52 kDa

Human KRT14 Western blot (WB) 13048

Anti-Cytokeratin14 rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:52 kDa

Anti-Cytokeratin 14 Antibody Alternative Names
Anti-CK14 Antibody;Anti-EBS3 Antibody;Anti-EBS4 Antibody;Anti-K14 Antibody;Anti-NFJ Antibody
Cytokeratin 14 Background Information

Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis.

Full Name
keratin 14, type I
References
  • Coulombe PA, et al., 1991, Cell. 66(6): 1301-11.
  • Schweizer J, et al., 2006, 174(2): 169-74.
  • Lugassy J, et al., 2006, Am J Hum Genet. 79(4): 724-30.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cytokeratin 14 Antibody Rabbit Polyclonal WB,IHC-P,IP 106198-T44
    Anti-Cytokeratin 14 Antibody Mouse Monoclonal WB 100129-MM02T
    Anti-Cytokeratin 14 Antibody Mouse Monoclonal IHC-P 100129-MM01T
    Anti-Cytokeratin 14 Antibody Rabbit Polyclonal WB,IHC-P 100129-RP02
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