|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
KRT14 was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-KRT14 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 52 kDa
Observed band size: 52 kDa
Anti-Cytokeratin14 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:52 kDa
Observed band size:52 kDa
Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Cytokeratin 14 Antibody||Rabbit||Polyclonal||WB,IHC-P,IP||106198-T44|
|Anti-Cytokeratin 14 Antibody||Mouse||Monoclonal||WB||100129-MM02T|
|Anti-Cytokeratin 14 Antibody||Mouse||Monoclonal||IHC-P||100129-MM01T|
|Anti-Cytokeratin 14 Antibody||Rabbit||Polyclonal||WB,IHC-P||100129-RP02|