Anti-Cystatin S Antibody

Cat: 11542-MM01
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Anti-Cystatin S Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Cystatin S Antibody
Validated applications
ELISA,ELISA(Cap)
Species reactivity
Reacts with: Human
Specificity
Human Cystatin S
No cross-reactivity with Human cell lysate (293 cell line) in ELISA.
Immunogen
Recombinant Human Cystatin S / CST4 Protein (Catalog#11542-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cystatin S / CST4 (rh Cystatin S / CST4; Catalog#11542-H08H; NP_001890.1; Met 1-Ala 141). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #9E2E2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cystatin S Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human Cystatin S / CST4 in ELISA pair set (Catalog: # SEK11542). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 11542-MM10).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cystatin S Antibody Alternative Names
Anti-CST4 Antibody;Anti-MGC71923 Antibody
Cystatin S Background Information

Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to the cystatin family. Cystatin-4 / CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4 / CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4 / CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.

Full Name
cystatin S
References
  • Bobek L.A., et al.,(1991), Human salivary cystatin S. Cloning, sequence analysis, hybridization in situ and immunocytochemistry. Biochem. J. 278:627-635.
  • Saitoh E., et al., (1992), Characterization of two members (CST4 and CST5) of the cystatin gene family and molecular evolution of cystatin genes.Agents Actions 38:340-348.
  • Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cystatin S Antibody Rabbit Monoclonal ELISA,FCM,ICC/IF,IF 11542-R006
    Anti-Cystatin S Antibody Mouse Monoclonal ELISA(Det) 11542-MM10
    Anti-Cystatin S Antibody Rabbit Polyclonal ELISA 11542-RP01
    Anti-Cystatin S Antibody Rabbit Polyclonal ELISA 11542-RP02
    Anti-Cystatin S Antibody Rabbit Polyclonal ELISA 11542-T16
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