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Human Cystatin 7 / CST7 ELISA Pair Set

  • Human Cystatin 7 / CST7 ELISA Pair Set Standard Curve 14827
  • Human Cystatin F / CST7 ELISA standard curve
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Human Cystatin F/CST7 Materials provided
Capture Ab:1 mg/mL of mouse anti-Human Cystatin 7 / CST7 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL of mouse anti-Human Cystatin 7 / CST7 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 180 ng of recombinant Human Cystatin 7 / CST7. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
Human Cystatin F/CST7 Sensitivity
The minimum detectable dose of Human Cystatin 7 / CST7 was determined to be approximately 234.4 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human Cystatin F/CST7 Principle of the product
The Human Cystatin 7 / CST7 ELISA Pair Set is for the quantitative determination of Human Cystatin 7 / CST7.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Cystatin 7 / CST7 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Cystatin 7 / CST7 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Cystatin 7 / CST7 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Cystatin 7 / CST7 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human Cystatin F/CST7 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human Cystatin F/CST7 ELISA Pair Set Standard Curve
Cystatin 7 / CST7 Background

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

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Datasheet & Documentation

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