Cystatin F Matched ELISA Antibody Pair Set,Human General Information
Cystatin F Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human Cystatin F
1. Capture Antibody 1 mg/mL of mouse anti-Human Cystatin 7 / CST7 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human Cystatin 7 / CST7 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 180 ng of recombinant Human Cystatin 7 / CST7. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
This Cystatin F Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Cystatin F . It contains Human Cystatin F capture antibody, Human Cystatin F detector antibody
and a highly purified
recombinant Human Cystatin F protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Cystatin F Matched ELISA Antibody Pair Set,Human Images
Cystatin F Matched ELISA Antibody Pair Set,Human Alternative Names
CMAP Matched ELISA Antibody Pair Set, Human
Cystatin F Background Information
The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.
cystatin F (leukocystatin)
Halfon S., et al.,(1998), Leukocystatin, a new class II cystatin expressed selectively by hematopoietic cells. J. Biol. Chem. 273:16400-16408.Ni J., et al., (1998), Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor.J. Biol. Chem. 273:24797-24804.Morita M., et al.,(2000), Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3).Genomics 67:87-91.