Mouse Cystatin-F / CST7 / Cystatin-7 Protein

Cystatin F (leukocystatin) Protein Datasheet

Cystatin-F / CST7 / Cystatin-7 Protein Product Information

• Synonym: CST7
• Protein Construction: A DNA sequence encoding the mouse CST7 (O89098) (Met1- Gln144) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

Cystatin-F / CST7 / Cystatin-7 Protein QC Testing

• Purity: > 93 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.
  The IC₅₀ is < 6 nM.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 19
• Molecular Mass: The recombinant mouse CST7 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 367 amino acids and has a predicted molecular mass of 41.4 KDa. The apparent molecular mass of the protein is approximately 42 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 25mMTris,0.15MNaCl,pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Cystatin-F / CST7 / Cystatin-7 protein

Cystatin-F / CST7 / Cystatin-7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cystatin-F / CST7 / Cystatin-7 Protein Description

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

References

1. Ni J. et al., 1998, J Biol Chem. 273: 24797-804.
2. Morita M. et al., 1999, Cancer Res. 59:151–8.
3. Langerholc T. et al., 2005, FEBS J. 272: 1535-45.
4. Hamilton G. et al., 2008, EMBO J. 27: 499-508.
5. Alexander W. et al., 2006, J Biol Chem. 281: 16570-5.