Anti-Cystatin F Antibody

Cat: 10167-MM01
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All Cystatin F Reagents
Anti-Cystatin F Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Cystatin F Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Cystatin F
Recombinant Human CST7 protein (Catalog#10167-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CST7 (rh CST7; Catalog#10167-H08H; NP_003641.2; Met 1-His 145). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #2F11D9B2
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cystatin F Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cystatin F Antibody (Mouse Monoclonal antibody) Images
Human Cystatin 7 / CST7 Western blot (WB) 13131

Anti-CST7 mouse monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:21 kDa

Observed band size:27 kDa

Anti-Cystatin F Antibody Alternative Names
Anti-CMAP Antibody
Cystatin F Background Information

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

Full Name
cystatin F (leukocystatin)
  • Halfon S., et al.,(1998), Leukocystatin, a new class II cystatin expressed selectively by hematopoietic cells. J. Biol. Chem. 273:16400-16408.
  • Ni J., et al., (1998), Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor.J. Biol. Chem. 273:24797-24804.
  • Morita M., et al.,(2000), Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3).Genomics 67:87-91.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cystatin F Antibody Rabbit Polyclonal ELISA 50236-RP01
    Anti-Cystatin F Antibody Rabbit Polyclonal ELISA 50236-T16
    Anti-Cystatin F Antibody Mouse Monoclonal ELISA 10167-MM04
    Anti-Cystatin F Antibody Rabbit Polyclonal ELISA 10167-RP02
    Anti-Cystatin F Antibody Rabbit Polyclonal ELISA 10167-RP01
    Anti-Cystatin F Antibody Rabbit Polyclonal WB 10167-T32
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