Mouse Cystatin-E / CST6 / Cystatin-M / Cystatin-6 Protein (His Tag)

Cystatin E/M Protein Datasheet

Cystatin-E / CST6 / Cystatin-M / Cystatin-6 Protein Product Information

• Synonym: 1110017E11Rik, N28197, ichq
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CST6 (NP_082899.1) (Met 1-Ala 152) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

Cystatin-E / CST6 / Cystatin-M / Cystatin-6 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 29
• Molecular Mass: The secreted recombinant mouse CST6 comprises 132 amino acids and has a calculated molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rmCST6 is approximately 20 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Cystatin-E / CST6 / Cystatin-M / Cystatin-6 protein

Cystatin-E / CST6 / Cystatin-M / Cystatin-6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cystatin-E / CST6 / Cystatin-M / Cystatin-6 Protein Description

Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.

References

1. Soutiropoulou G. et al., 1997, J Biol Chem. 272: 903-10.
2. Zhang J. et al., 2004, Cancer Res. 64: 6957-64.
3. Zeeuwen PL. et al., 2003, J Invest Dermatol. 121: 65-8.
4. Schagdarsurengin U. et al., 2007, Oncogene. 26: 3089-94.
5. Cheng T. et al., 2006, J Biol Chem. 281: 15893-9.