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Human CystatinC / CST3 Gene cDNA Clone (full-length ORF Clone)
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Human CystatinC / CST3 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10439-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000099.2

cDNA Size:   441

Synonym:  ARMD11; MGC117328; CST3

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens cystatin C DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 73G/A resulting in the amino acid Ala substitution by Thr.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC
61       GTGAGCCCCG CGACCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
121      GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
181      AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
241      CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
301      AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
361      GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
421      TCCACCTGTC AGGACGCCTA G


Quality Control:   cDNA sequencing results is available upon request.

 

Cystatin C(CST3) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"