|IP||2-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CST3 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate2 µL anti-CST3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CST3 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 14 kDa
Observed band size: 14 kDa
Anti-CST3 rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:13 kDa
Observed band size:13 kDa
Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Cystatin C Antibody||Rabbit||Polyclonal||ELISA||80040-RP01|
|Anti-Cystatin C Antibody||Rabbit||Polyclonal||ELISA,IHC-P||80040-RP02|
|Anti-Cystatin C Antibody||Rabbit||Monoclonal||ELISA||80040-R002|
|Anti-Cystatin C Antibody||Rabbit||Monoclonal||IHC-P||80040-R001|
|Anti-Cystatin C Antibody||Rabbit||Polyclonal||WB,ELISA,IP||50239-RP02|
|Anti-Cystatin C Antibody||Rabbit||Polyclonal||ELISA||50239-RP01|
|Anti-Cystatin C Antibody||Rabbit||Monoclonal||ELISA||50239-R041|
|Anti-Cystatin C Antibody||Mouse||Monoclonal||ELISA(Det)||10439-MM06|
|Anti-Cystatin C Antibody||Mouse||Monoclonal||ELISA||10439-MM13|
|Anti-Cystatin C Antibody||Rabbit||Monoclonal||IHC-P||10439-R033|
|Anti-Cystatin C Antibody||Rabbit||Polyclonal||ELISA||10439-RP01|
|Anti-Cystatin C Antibody||Mouse||Monoclonal||ELISA(Cap)||10439-MM03|
|Anti-Cystatin C Antibody||Rabbit||Monoclonal||ELISA||10439-R001|