Anti-Cystatin C Antibody

Cat: 10439-RP02
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Anti-Cystatin C Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cystatin C Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Cystatin C
Immunogen
Recombinant Human Cystatin C / CST3 protein (Catalog#10439-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Cystatin C / CST3 (rh Cystatin C / CST3; Catalog#10439-H08H; NP_000090.1; Ser 27-Ala 146). Cystatin C / CST3 specific IgG was purified by Human Cystatin C / CST3 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cystatin C Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 2-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cystatin C Antibody (Rabbit Polyclonal antibody) Images
Human Cystatin C / CST3 Immunoprecipitation(IP) 7985

CST3 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-CST3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CST3 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 14 kDa

Observed band size: 14 kDa

Human Cystatin C / CST3 Western blot (WB) 13218

Anti-CST3 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:13 kDa

Observed band size:13 kDa

Anti-Cystatin C Antibody Alternative Names
Anti-ARMD11 Antibody
Cystatin C Background Information

Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.

Full Name
cystatin C
References
  • Mares J, et al. (2003) Use of cystatin C determination in clinical diagnostics. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 147(2): 177-80.
  • Mussap M, et al. (2004) Biochemistry and clinical role of human cystatin C. Crit Rev Clin Lab Sci. 41(5-6): 467-550.
  • Sronie-Vivien S, et al. (2008) Cystatin C: current position and future prospects. Clin Chem Lab Med. 46(12): 1664-86.
  • Taglieri N, et al. (2009) Cystatin C and cardiovascular risk. Clin Chem. 55(11): 1932-43.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cystatin C Antibody Rabbit Polyclonal ELISA 80040-RP01
    Anti-Cystatin C Antibody Rabbit Monoclonal IHC-P 80040-R001
    Anti-Cystatin C Antibody Rabbit Polyclonal ELISA,IHC-P 80040-RP02
    Anti-Cystatin C Antibody Rabbit Monoclonal ELISA 80040-R002
    Anti-Cystatin C Antibody Rabbit Polyclonal WB,ELISA,IP 50239-RP02
    Anti-Cystatin C Antibody Rabbit Monoclonal ELISA 50239-R041
    Anti-Cystatin C Antibody Rabbit Polyclonal ELISA 50239-RP01
    Anti-Cystatin C Antibody Rabbit Monoclonal ELISA 10439-R001
    Anti-Cystatin C Antibody Mouse Monoclonal ELISA(Det) 10439-MM06
    Anti-Cystatin C Antibody Mouse Monoclonal ELISA 10439-MM13
    Anti-Cystatin C Antibody Mouse Monoclonal ELISA(Cap) 10439-MM03
    Anti-Cystatin C Antibody Rabbit Monoclonal IHC-P 10439-R033
    Anti-Cystatin C Antibody Rabbit Polyclonal ELISA 10439-RP01
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