Cystatin C / CST3 Antibody

Cystatin C / CST3 Antibody Datasheet

Cystatin C / CST3 Antibody Product Information

• Immunogen: Recombinant Human Cystatin C / CST3 protein (Catalog#10439-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Cystatin C / CST3 (rh Cystatin C / CST3; Catalog#10439-H08H; NP_000090.1; Met 1-Ala 146). Total IgG was purified by Protein A affinity chromatography.

Cystatin C / CST3 Antibody Usage Guide

• Specificity: Human Cystatin C / CST3
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CST3 in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CST3. The detection limit for Human CST3 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cystatin C / CST3 Antibody Background

Cystatin-C / CST3 is a secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily, and exerts actions on a variety of cysteine proteases including papain and Cathepsins B, H, K, L and S. As the most abundant extracellular inhibitor of cysteine proteases, Cystatin-C encoded by a housekeeping gene is found ubiquitously in all organs and biological fluids at high concentration levels. It has been shown that cystatin-C binds amyloid-beta and reduces cerebral amyloid-beta deposition or inhibits its fibril formation in an APP-transgenic mouse model. Thus, a point mutation in this gene (Leu 68 to Gln) hsa been associated with a severe hereditary form of amyloid angiopathy. Studies have also investigated cystatin-C as a marker of kidney function and thyroid dysfunction prior to creatinine, and could be a useful clinical tool to identify HIV-infected persons. In addition, cystatin-C expression is up-regulated in malignance of certain tumor progression.

References

1. Janowski, R. et al., 2001, Nat. Struct. Biol. 8: 316-320.
2. Kos, J. et al., 1998, Clin.Chem. 44: 2556-2557.
3. Fricker, M. et al., 2003, Kidney. Int. 63: 1944-1947.
4. Kaeser, S.A. et al., 2007, Nat. Genet. 39: 1437-1439.
5. Odden, M.C. et al., 2007, Arch. Intern. Med. 167: 2213-2219.