Cystatin C / CST3 Antibody (Antigen Affinity Purified)

Cystatin C / CST3 Antibody ( Antigen Affinity Purified ) Datasheet

Cystatin C / CST3 Antibody Product Information

• Immunogen: Recombinant Rat Cystatin C / CST3 protein (Catalog#80040-R08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose

Cystatin C / CST3 Antibody Usage Guide

• Specificity: Rat Cystatin C / CST3
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of rat CST3 in rat brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of rat CST3 in rat kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  

  Immunochemical staining of rat CST3 in rat testis with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CST3 in WB. Using a DAB detection system, the detection limit for Rat CST3 is approximately 4 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CST3. The detection limit for Rat CST3 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cystatin C / CST3 Antibody Background

Cystatin-C / CST3 is a secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily, and exerts actions on a variety of cysteine proteases including papain and Cathepsins B, H, K, L and S. As the most abundant extracellular inhibitor of cysteine proteases, Cystatin-C encoded by a housekeeping gene is found ubiquitously in all organs and biological fluids at high concentration levels. It has been shown that cystatin-C binds amyloid-beta and reduces cerebral amyloid-beta deposition or inhibits its fibril formation in an APP-transgenic mouse model. Thus, a point mutation in this gene (Leu 68 to Gln) hsa been associated with a severe hereditary form of amyloid angiopathy. Studies have also investigated cystatin-C as a marker of kidney function and thyroid dysfunction prior to creatinine, and could be a useful clinical tool to identify HIV-infected persons. In addition, cystatin-C expression is up-regulated in malignance of certain tumor progression.

References

1. Janowski, R. et al., 2001, Nat. Struct. Biol. 8: 316-320.
2. Kos, J. et al., 1998, Clin.Chem. 44: 2556-2557.
3. Fricker, M. et al., 2003, Kidney. Int. 63: 1944-1947.
4. Kaeser, S.A. et al., 2007, Nat. Genet. 39: 1437-1439.
5. Odden, M.C. et al., 2007, Arch. Intern. Med. 167: 2213-2219.