Anti-Cystatin A Antibody

Cat: 10479-RP01
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Anti-Cystatin A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cystatin A Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Cystatin A
Immunogen
Recombinant Human Cystatin A / CSTA protein (Catalog#10479-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Cystatin A / CSTA (rh Cystatin A / CSTA; Catalog#10479-H07E; P01040; Ile 2-Phe 98). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cystatin A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cystatin A Antibody (Rabbit Polyclonal antibody) Images
Human Cystatin A / CSTA Immunohistochemistry(IHC) 4337
Immunochemical staining of human CSTA in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-Cystatin A Antibody Alternative Names
Anti-AREI Antibody;Anti-STF1 Antibody;Anti-STFA Antibody
Cystatin A Background Information

Cystatin-A, also known as Cystatin-AS, Stefin-A and CSTA, is a cytoplasm protein which belongs to the cystatin family. Cystatin-A / CSTA is a cysteine proteinase inhibitor with a molecular mass of 11 kDa, and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Full Name
cystatin A
References
  • Machleidt W., et al.,(1983), Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe-Seyler's Z. Physiol. Chem. 364:1481-1486.
  • Takeda A., et al., (1989), Comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes.J. Biochem. 105:986-991.
  • Kartasova T., et al.,(1987), Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.Nucleic Acids Res. 15:5945-5962.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cystatin A Antibody Rabbit Polyclonal ELISA,IHC-P 10479-T24
    Anti-Cystatin A Antibody Rabbit Polyclonal ELISA,IHC-P 10479-RP02
    Anti-Cystatin A Antibody Rabbit Monoclonal WB 10479-R236
    Anti-Cystatin A Antibody Mouse Monoclonal IHC-P 10479-MM01
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