|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus IL1A ORF mammalian expression plasmid, C-GFPSpark tag||CG90124-ACG|
|Rhesus IL1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90124-ACR|
|Rhesus IL1A ORF mammalian expression plasmid, C-Flag tag||CG90124-CF|
|Rhesus IL1A ORF mammalian expression plasmid, C-His tag||CG90124-CH|
|Rhesus IL1A ORF mammalian expression plasmid, C-Myc tag||CG90124-CM|
|Rhesus IL1A ORF mammalian expression plasmid, C-HA tag||CG90124-CY|
|Rhesus IL1A Gene cDNA clone plasmid||CG90124-G|
|Rhesus IL1A ORF mammalian expression plasmid, N-Flag tag||CG90124-NF|
|Rhesus IL1A ORF mammalian expression plasmid, N-His tag||CG90124-NH|
|Rhesus IL1A ORF mammalian expression plasmid, N-Myc tag||CG90124-NM|
|Rhesus IL1A ORF mammalian expression plasmid, N-HA tag||CG90124-NY|
|Rhesus IL1A natural ORF mammalian expression plasmid||CG90124-UT|
|Learn more about expression Vectors|
IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.