|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus HSPA8 ORF mammalian expression plasmid, C-GFPSpark tag||CG90471-ACG|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90471-ACR|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-Flag tag||CG90471-CF|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-His tag||CG90471-CH|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-Myc tag||CG90471-CM|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-HA tag||CG90471-CY|
|Rhesus HSPA8 Gene cDNA clone plasmid||CG90471-G|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-Flag tag||CG90471-NF|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-His tag||CG90471-NH|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-Myc tag||CG90471-NM|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-HA tag||CG90471-NY|
|Rhesus HSPA8 natural ORF mammalian expression plasmid||CG90471-UT|
|Learn more about expression Vectors|
HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.