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Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-His tag

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Cynomolgus GOT1 cDNA Clone Product Information
NCBI RefSeq:NM_001284930.1
RefSeq ORF Size:1242bp
cDNA Description:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Aspartate aminotransferase, cytoplasmic with N terminal His tag.
Gene Synonym:cCAT, cAspAT
Species:Cynomolgus
Vector:pCMV3-N-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-His tag on other vectors
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagCG90649-ACG$225
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagCG90649-ACR$225
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagCG90649-ANG$225
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagCG90649-ANR$225
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Flag tagCG90649-CF$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-His tagCG90649-CH$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Myc tagCG90649-CM$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-HA tagCG90649-CY$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone in cloning vectorCG90649-G$75
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Flag tagCG90649-NF$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-His tagCG90649-NH$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Myc tagCG90649-NM$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-HA tagCG90649-NY$195
Cynomolgus Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmidCG90649-UT$195
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Background

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

References
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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    Catalog: CG90649-NH
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