SMAC cDNA ORF Clone, Cynomolgus, N-His tag

Cat: CG90706-NH
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All SMAC Reagents
CRO Services
Product recommended

All SMAC Reagents
SMAC cDNA ORF Clone, Cynomolgus, N-His tag General Information
Gene
Species
Cynomolgus
NCBI Ref Seq
RefSeq ORF Size
561 bp
Description
Full length Clone DNA of Cynomolgus diablo, IAP-binding mitochondrial protein with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-His
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SMAC Background Information

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

Full Name
diablo, IAP-binding mitochondrial protein
References
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
  • SMAC cDNA ORF Clone, Cynomolgus, Related Products

    Product Name Catalog#(PDF)
    SMAC cDNA ORF Clone in Cloning Vector, Cynomolgus CG90706-G
    SMAC cDNA ORF Clone, Cynomolgus, untagged CG90706-UT
    SMAC cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90706-ACG
    SMAC Lentiviral cDNA ORF Clone, Cynomolgus, C-GFPSpark® tag CG90706-ACGLN
    SMAC cDNA ORF Clone, Cynomolgus, N-GFPSpark® tag CG90706-ANG
    SMAC cDNA ORF Clone, Cynomolgus, C-OFPSpark® tag CG90706-ACR
    SMAC cDNA ORF Clone, Cynomolgus, N-OFPSpark® tag CG90706-ANR
    SMAC cDNA ORF Clone, Cynomolgus, C-DDK (Flag®) tag CG90706-CF
    SMAC cDNA ORF Clone, Cynomolgus, N-DDK (Flag®) tag CG90706-NF
    SMAC cDNA ORF Clone, Cynomolgus, C-His tag CG90706-CH
    SMAC cDNA ORF Clone, Cynomolgus, C-HA tag CG90706-CY
    SMAC cDNA ORF Clone, Cynomolgus, N-HA tag CG90706-NY
    SMAC cDNA ORF Clone, Cynomolgus, C-Myc tag CG90706-CM
    SMAC cDNA ORF Clone, Cynomolgus, N-Myc tag CG90706-NM

    SMAC cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    SMAC cDNA ORF Clone in Cloning Vector, Mouse MG53841-U
    SMAC cDNA ORF Clone, Mouse, untagged MG53841-UT
    SMAC cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53841-ACG
    SMAC Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53841-ACGLN
    SMAC cDNA ORF Clone, Mouse, N-GFPSpark® tag MG53841-ANG
    SMAC cDNA ORF Clone, Mouse, C-OFPSpark® tag MG53841-ACR
    SMAC cDNA ORF Clone, Mouse, N-OFPSpark® tag MG53841-ANR
    SMAC cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53841-CF
    SMAC cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG53841-NF
    SMAC cDNA ORF Clone, Mouse, C-His tag MG53841-CH
    SMAC cDNA ORF Clone, Mouse, N-His tag MG53841-NH
    SMAC cDNA ORF Clone, Mouse, C-HA tag MG53841-CY
    SMAC cDNA ORF Clone, Mouse, N-HA tag MG53841-NY
    SMAC cDNA ORF Clone, Mouse, C-Myc tag MG53841-CM
    SMAC cDNA ORF Clone, Mouse, N-Myc tag MG53841-NM

    SMAC cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    SMAC cDNA ORF Clone in Cloning Vector, Human HG10339-M
    SMAC cDNA ORF Clone, Human, untagged HG10339-UT
    SMAC cDNA ORF Clone, Human, C-GFPSpark® tag HG10339-ACG
    SMAC Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10339-ACGLN
    SMAC cDNA ORF Clone, Human, N-GFPSpark® tag HG10339-ANG
    SMAC cDNA ORF Clone, Human, C-OFPSpark® tag HG10339-ACR
    SMAC cDNA ORF Clone, Human, N-OFPSpark® tag HG10339-ANR
    SMAC cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10339-CF
    SMAC cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10339-NF
    SMAC cDNA ORF Clone, Human, C-His tag HG10339-CH
    SMAC cDNA ORF Clone, Human, N-His tag HG10339-NH
    SMAC cDNA ORF Clone, Human, C-HA tag HG10339-CY
    SMAC cDNA ORF Clone, Human, N-HA tag HG10339-NY
    SMAC cDNA ORF Clone, Human, C-Myc tag HG10339-CM
    SMAC cDNA ORF Clone, Human, N-Myc tag HG10339-NM
    SMAC qPCR Primer Pairs, Human HP100033
    info info
    添加购物车成功! 添加购物车失败!