|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||CG90188-ACG|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||CG90188-ACR|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-Flag tag||CG90188-CF|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-His tag||CG90188-CH|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-Myc tag||CG90188-CM|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, C-HA tag||CG90188-CY|
|Rhesus CD59 Gene ORF cDNA clone in cloning vector||CG90188-G|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, N-Flag tag||CG90188-NF|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, N-His tag||CG90188-NH|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, N-Myc tag||CG90188-NM|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid, N-HA tag||CG90188-NY|
|Rhesus CD59 Gene ORF cDNA clone expression plasmid||CG90188-UT|
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CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).