|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus CD40LG ORF mammalian expression plasmid, C-GFPSpark tag||CG90096-ACG|
|Rhesus CD40LG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90096-ACR|
|Rhesus CD40LG ORF mammalian expression plasmid, C-Flag tag||CG90096-CF|
|Rhesus CD40LG ORF mammalian expression plasmid, C-His tag||CG90096-CH|
|Rhesus CD40LG ORF mammalian expression plasmid, C-Myc tag||CG90096-CM|
|Rhesus CD40LG ORF mammalian expression plasmid, C-HA tag||CG90096-CY|
|Rhesus CD40LG Gene cDNA clone plasmid||CG90096-G|
|Rhesus CD40LG ORF mammalian expression plasmid, N-Flag tag||CG90096-NF|
|Rhesus CD40LG ORF mammalian expression plasmid, N-His tag||CG90096-NH|
|Rhesus CD40LG ORF mammalian expression plasmid, N-Myc tag||CG90096-NM|
|Rhesus CD40LG ORF mammalian expression plasmid, N-HA tag||CG90096-NY|
|Rhesus CD40LG natural ORF mammalian expression plasmid||CG90096-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.