|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus AGRP ORF mammalian expression plasmid, C-GFPSpark tag||CG90196-ACG|
|Rhesus AGRP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90196-ACR|
|Rhesus AGRP ORF mammalian expression plasmid, C-Flag tag||CG90196-CF|
|Rhesus AGRP ORF mammalian expression plasmid, C-His tag||CG90196-CH|
|Rhesus AGRP ORF mammalian expression plasmid, C-Myc tag||CG90196-CM|
|Rhesus AGRP ORF mammalian expression plasmid, C-HA tag||CG90196-CY|
|Rhesus AGRP Gene cDNA clone plasmid||CG90196-G|
|Rhesus AGRP ORF mammalian expression plasmid, N-Flag tag||CG90196-NF|
|Rhesus AGRP ORF mammalian expression plasmid, N-His tag||CG90196-NH|
|Rhesus AGRP ORF mammalian expression plasmid, N-Myc tag||CG90196-NM|
|Rhesus AGRP ORF mammalian expression plasmid, N-HA tag||CG90196-NY|
|Rhesus AGRP natural ORF mammalian expression plasmid||CG90196-UT|
|Learn more about expression Vectors|
Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.