Anti-Cyclophilin B Antibody

Cat: 101242-T36
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Anti-Cyclophilin B Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cyclophilin B Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Cyclophilin B
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Cyclophilin B / PPIB
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Cyclophilin B / PPIB, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cyclophilin B Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cyclophilin B Antibody (Rabbit Polyclonal antibody) Images
Human Cyclophilin B / PPIB Immunoprecipitation(IP) 6005

PPIB was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-PPIB rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-PPIB rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 19 kDa

Observed band size: 19 kDa

Human Cyclophilin B / PPIB Western blot (WB) 6571

Anti-PPIB rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela whole cell lysate

Lane B: A431 whole cell lysate

Lane C: HepG2 whole cell lysate

Lane D: 293 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:19 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cyclophilin B Antibody Alternative Names
Anti-CYP-S1 Antibody;Anti-CYPB Antibody;Anti-HEL-S-39 Antibody;Anti-OI9 Antibody;Anti-SCYLP Antibody
Cyclophilin B Background Information
Full Name
peptidylprolyl isomerase B (cyclophilin B)
Product Description Host Clonality Application Catalog# (PDF)
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