Mouse CCNE1 Gene cDNA Clone / ORF Clone

CCNE1 cDNA Clone Datasheet

CCNE1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007633.2
• cDNA Size: 1227
• Gene Synonym: AW538188; Ccne1
• Source: Mouse

CCNE1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin E1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for four point mutations: 537 T/G resulting in the amino acid His substitution by Gln, 258 C/T, 402 T/C, 450 A/G not causing the amino acid variation.

CCNE1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCNE1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCNE1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCAAGGG AGAGAGACTC GACGGACCAC AGCAACATGA AAGAAGAAGG TGGCTCCGAC
61       CTTTCAGTCC GCTCCAGAAA AAGGAAGGCA AATGTGGCCG TGTTTTTGCA AGACCCAGAT
121      GAAGAAATTG CCAAGATTGA CAAGACTGTG AAAAGCGAGG ATAGCAGTCA GCCCTGGGAT
181      GATAATTCAG CATGCGTGGA CCCCTGCTCT TTCATCCCCA CCCCTAACAA AGAAGAGGAC
241      AATGAGCTTG AATACCCTAG GACTGCATTT CAGCCTCGGA AAATCAGACC ACCCAGAGCC
301      TCCCCACTTC CCGTCTTGAA TTGGGGCAAT AGAGAAGAGG TTTGGAGGAT CATGTTAAAC
361      AAAGAAAAGA CTTACCTGAG AGATGAGCAC TTTCTGCAGC GCCATCCTCT CCTGCAGGCG
421      AGGATGAGAG CAGTTCTTCT GGATTGGCTG ATGGAGGTGT GCGAAGTCTA TAAGCTCCAC
481      AGAGAGACGT TCTACTTGGC ACAGGACTTC TTTGATCGTT ACATGGCATC ACAACAGAAT
541      ATCATAAAAA CACTTTTACA GCTTATTGGG ATTTCAGCCT TATTTATTGC TTCAAAACTT
601      GAGGAAATCT ACCCTCCAAA GTTGCACCAG TTTGCTTATG TTACAGATGG CGCTTGCTCC
661      GGGGATGAAA TTCTTACCAT GGAATTGATG ATGATGAAGG CCCTTAAGTG GCGTCTAAGC
721      CCTCTGACCA TTGTGTCCTG GCTGAATGTC TATGTCCAAG TGGCCTATGT CAACGACACG
781      GGTGAGGTGC TGATGCCTCA GTACCCACAG CAGGTCTTCG TGCAGATCGC AGAGCTTCTA
841      GACCTGTGCG TCCTGGATGT TGGCTGCTTA GAATTTCCTT ATGGTGTCCT CGCTGCTTCT
901      GCTTTGTATC ATTTCTCCTC ACTGGAGTTG ATGCAGAAGG TCTCAGGTTA TCAGTGGTGC
961      GACATAGAGA AGTGTGTCAA ATGGATGGTT CCGTTCGCCA TGGTTATCCG GGAGATGGGA
1021     AGTTCCAAGC TCAAGCACTT CCGGGGAGTC CCCATGGAAG ACTCCCACAA CATCCAGACC
1081     CACACCAACA GCTTGGATTT GCTGGACAAA GCCCAAGCAA AGAAAGCCAT ATTGTCAGAA
1141     CAGAATAGGA TTTCTCCTCC TCCGAGTGTG GTCCTGACAC CCCCACCCAG CAGTAAGAAG
1201     CAGAGCAGCG AGCAGGAGAC AGAATGA

 

Quality Control:cDNA sequencing results is available upon request.