Mouse CCND1 Gene cDNA Clone / ORF Clone

CCND1 cDNA Clone Datasheet

CCND1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007631.2
• cDNA Size: 888
• Gene Synonym: cD1; Cyl-1; PRAD1; bcl-1; AI327039; Ccnd1
• Source: Mouse

CCND1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin D1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 280 T/C not causing the amino acid variation.

CCND1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCND1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCND1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACACC AGCTCCTGTG CTGCGAAGTG GAGACCATCC GCCGCGCGTA CCCTGACACC
61       AATCTCCTCA ACGACCGGGT GCTGCGAGCC ATGCTCAAGA CGGAGGAGAC CTGTGCGCCC
121      TCCGTATCTT ACTTCAAGTG CGTGCAGAAG GAGATTGTGC CATCCATGCG GAAAATCGTG
181      GCCACCTGGA TGCTGGAGGT CTGTGAGGAG CAGAAGTGCG AAGAGGAGGT CTTCCCGCTG
241      GCCATGAACT ACCTGGACCG CTTCCTGTCC CTGGAGCCCC TGAAGAAGAG CCGCCTGCAG
301      CTGCTGGGGG CCACCTGCAT GTTCGTGGCC TCTAAGATGA AGGAGACCAT TCCCTTGACT
361      GCCGAGAAGT TGTGCATCTA CACTGACAAC TCTATCCGGC CCGAGGAGCT GCTGCAAATG
421      GAACTGCTTC TGGTGAACAA GCTCAAGTGG AACCTGGCCG CCATGACTCC CCACGATTTC
481      ATCGAACACT TCCTCTCCAA AATGCCAGAG GCGGATGAGA ACAAGCAGAC CATCCGCAAG
541      CATGCACAGA CCTTTGTGGC CCTCTGTGCC ACAGATGTGA AGTTCATTTC CAACCCACCC
601      TCCATGGTAG CTGCTGGGAG CGTGGTGGCT GCGATGCAAG GCCTGAACCT GGGCAGCCCC
661      AACAACTTCC TCTCCTGCTA CCGCACAACG CACTTTCTTT CCAGAGTCAT CAAGTGTGAC
721      CCGGACTGCC TCCGTGCCTG CCAGGAACAG ATTGAAGCCC TTCTGGAGTC AAGCCTGCGC
781      CAGGCCCAGC AGAACGTCGA CCCCAAGGCC ACTGAGGAGG AGGGGGAAGT GGAGGAAGAG
841      GCTGGTCTGG CCTGCACGCC CACCGACGTG CGAGATGTGG ACATCTGA

 

Quality Control:cDNA sequencing results is available upon request.