CCNA1 / Cyclin-A1 Protein (His Tag)

CCNA1 / Cyclin-A1 Protein Datasheet

CCNA1 / Cyclin-A1 Protein Product Information

• Synonym: CCNA1
• Protein Construction: A DNA sequence encoding the full length of human CCNA1 isoform a ( NP_003905.1 ) ( Met 1 - Gln 465 ) was expressed, with a polyhistidine tag at the N-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

CCNA1 / Cyclin-A1 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCNA1 protein

CCNA1 / Cyclin-A1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCNA1 / Cyclin-A1 Protein Description

Cyclin-A1, also known as CCNA1, is a nucleus protein which belongs to the cyclin family and Cyclin AB subfamily. The expression of Cyclin-A1 (CCNA1) is increased in early G1 phase and reaches highest levels during the S and G2/M phases. The mammalian A-type cyclin family consists of two members, Cyclin-A1 (CCNA1) and Cyclin-A2 (CCNA2). CCNA2 promotes both G1/S and G2/M transitions, and targeted deletion of Ccna2 in mouse is embryonic lethal Cyclin-A1 (CCNA1) is expressed in mice exclusively in the germ cell lineage and is expressed in humans at highest levels in the testis and certain myeloid leukaemia cells. Cyclin-A1 (CCNA1) is essential for spermatocyte passage into the first meiotic division in male mice, a function that cannot be complemented by the concurrently expressed B-type cyclins. Cyclin-A1 (CCNA1) may be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. Cyclin-A1 (CCNA1) may function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Cyclin-A1 (CCNA1) interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex

References

1. Yang R., et al., 1997, Cancer Res. 57: 913-20.
2. Liu, D. et al., 1998, Nat Genet. 20 (4): 377-80.
3. Yang R., et al., 1999, Mol. Cell. Biol. 19: 2400-07
4. Diederichs S., et al., 2004, J. Biol. Chem. 279: 33727-41.