CCNA1 / Cyclin-A1 Antibody

Cyclin-A1 / CCNA1 Antibody

Cyclin-A1 / CCNA1 Antibody Product Information

• Immunogen: Recombinant human CCNA1 protein ( Catalog#11013-H07B )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3F6B2C2
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human CCNA1 ( rh CCNA1 ; Catalog#11013-H07B ; NP_003905.1 ; Met 1 - Gln 465 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography

Cyclin-A1 / CCNA1 Antibody Usage Guide

• Specificity: Human CCNA1 / Cyclin-A1
• No cross-reactivity with Baculovirus lysate in ELISA
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CCNA1 in WB. Using a DAB detection system, the detection limit for human CCNA1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CCNA1. The detection limit for human CCNA1 is 0.0195 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cyclin-A1 / CCNA1 Antibody Background

Cyclin-A1, also known as CCNA1, is a nucleus protein which belongs to the cyclin family and Cyclin AB subfamily. The expression of Cyclin-A1 (CCNA1) is increased in early G1 phase and reaches highest levels during the S and G2/M phases. The mammalian A-type cyclin family consists of two members, Cyclin-A1 (CCNA1) and Cyclin-A2 (CCNA2). CCNA2 promotes both G1/S and G2/M transitions, and targeted deletion of Ccna2 in mouse is embryonic lethal Cyclin-A1 (CCNA1) is expressed in mice exclusively in the germ cell lineage and is expressed in humans at highest levels in the testis and certain myeloid leukaemia cells. Cyclin-A1 (CCNA1) is essential for spermatocyte passage into the first meiotic division in male mice, a function that cannot be complemented by the concurrently expressed B-type cyclins. Cyclin-A1 (CCNA1) may be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. Cyclin-A1 (CCNA1) may function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Cyclin-A1 (CCNA1) interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex

References

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3. Yang R., et al., 1999, Mol. Cell. Biol. 19: 2400-07.
4. Diederichs S., et al., 2004, J. Biol. Chem. 279: 33727-41.