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Human TDGF1 Gene cDNA Clone / ORF Clone

Catalog Size (Price) Quantity In Stock Operation Other Information
HG10908-M
  YES          

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_003212.2

cDNA Size:   567

Synonym:  CR, CRGF, CRIPTO, Cripto-1

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens teratocarcinoma-derived growth factor 1 DNA.

Vector:  Vector pMD18-T Simple

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations:65T/C resulting in the amino acid 22Val substitution by Ala,and 322C/T resulting in the amino acid 108His substitution by Tyr.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Remove the marked edge in the center of the spot .
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95℃ for 15-30 min.
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGACTGCA GGAAGATGGC CCGCTTCTCT TACAGTGTGA TTTGGATCAT GGCCATTTCT
61       AAAGCCTTTG AACTGGGATT AGTTGCCGGG CTGGGCCATC AGGAATTTGC TCGTCCATCT
121      CGGGGATACC TGGCCTTCAG AGATGACAGC ATTTGGCCCC AGGAGGAGCC TGCAATTCGG
181      CCTCGGTCTT CCCAGCGTGT GCCGCCCATG GGGATACAGC ACAGTAAGGA GCTAAACAGA
241      ACCTGCTGCC TGAATGGGGG AACCTGCATG CTGGGGTCCT TTTGTGCCTG CCCTCCCTCC
301      TTCTACGGAC GGAACTGTGA GTACGATGTG CGCAAAGAGA ACTGTGGGTC TGTGCCCCAT
361      GACACCTGGC TGCCCAAGAA GTGTTCCCTG TGTAAATGCT GGCACGGTCA GCTCCGCTGC
421      TTTCCTCAGG CATTTCTACC CGGCTGTGAT GGCCTTGTGA TGGATGAGCA CCTCGTGGCT
481      TCCAGGACTC CAGAACTACC ACCGTCTGCA CGTACTACCA CTTTTATGCT AGTTGGCATC
541      TGCCTTTCTA TACAAAGCTA CTATTAA


Quality Control:   cDNA sequencing results is available upon request.

 

Cripto/TDGF1 related areas, pathways, and other information