CNTN5 / Contactin-5 Antibody

Contactin-5 / CNTN5 Antibody

Contactin-5 / CNTN5 Antibody Product Information

• Immunogen: Recombinant human CNTN5 protein ( Catalog#10513-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 121
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CNTN5 / Contactin-5 ( rh CNTN5; Catalog#10513-H08H ; Met 1 - Gln 1059 ; NP_055176.1 ).

Contactin-5 / CNTN5 Antibody Usage Guide

• Specificity: Human CNTN5 / Contactin-5
• No cross-reactivity in ELISA with

  Human Contactin-2 / CNTN2 / TAG-1

  Human CNTN3 / Contactin-3 / BIG-1

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CNTN5 in WB. Using a DAB detection system, the detection limit for human CNTN5 is approximately 0.5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CNTN5. The detection limit for human CNTN5 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Contactin-5 / CNTN5 Antibody Background

Human Contactin-5 ( CNTN5 ), also known as NB-2, is an membrane-anchored neuronal protein and also an adhesion molecule. CNTN5 is one of the neural recognition molecules in the contactin subgroup, which belongs to the immunoglobulin superfamily. CNTN5 shared 46, 43, 55 and 55% identities with CNTN1, CNTN2, CNTN3, CNTN4 respectively at the amino acid sequence level. Expression of NB-2 mRNA was restricted to cerebrum, cerebellum and was hardlty detectable, if any, in spinal cord. In humans, two isoforms have been identified. The long isoform of human NB-2 consists of 1100 amino acids residues and the short isoform lacks 74 amino acid residues between residues 19 and 93 of the long isoform. CNTN5 may play a role in the developing nervous system. NB-2 promoted neurite outgrowth of the cerebral cortical neurons but not the hippocampal neurons. CNTN5-deficient mice demonstrate impaired neuronal activity in the auditory system, suggesting that it plays specific roles in the development and/or maturation of the regions.

References

1. Soumya R, et al., 2009, PLoS Genetics, Volume 5, Issue 6: e1000534
2. Yoshimasa Kamei, et al., 2000, Genomics 69: 113-119.
3. Junko O, et al., 1996, Neuroscience Letters, 218: 173-176.
4. Junko O, et al., 2001, Journal of Neuroscience Research, 65:100-110.
5. P. S. Walmod, et al., 2007, Handbook of Neurochemistry and Molecular Neurobiology.