Human Complement component 5/C5 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13416-G	Cloning vector ($395) pCMV / hygro ($645) pCMV / hygro - HA ($645) pCMV / hygro - His ($645) pCMV / hygro - Myc ($645) pCMV / hygro - FLAG ($645)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

C5 / complement component 5 cDNA Clone Datasheet

C5 / complement component 5 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001735.2
cDNA Size:	5031
Gene Synonym:	CPAMD4; C5
Source:	Human

C5 / complement component 5 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens complement component 5 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 4266 G/A not causing the amino acid variation.

C5 / complement component 5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C5 / complement component 5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C5 / complement component 5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCCTTT TGGGAATACT TTGTTTTTTA ATCTTCCTGG GGAAAACCTG GGGACAGGAG
61       CAAACATATG TCATTTCAGC ACCAAAAATA TTCCGTGTTG GAGCATCTGA AAATATTGTG
121      ATTCAAGTTT ATGGATACAC TGAAGCATTT GATGCAACAA TCTCTATTAA AAGTTATCCT
181      GATAAAAAAT TTAGTTACTC CTCAGGCCAT GTTCATTTAT CCTCAGAGAA TAAATTCCAA
241      AACTCTGCAA TCTTAACAAT ACAACCAAAA CAATTGCCTG GAGGACAAAA CCCAGTTTCT
301      TATGTGTATT TGGAAGTTGT ATCAAAGCAT TTTTCAAAAT CAAAAAGAAT GCCAATAACC
361      TATGACAATG GATTTCTCTT CATTCATACA GACAAACCTG TTTATACTCC AGACCAGTCA
421      GTAAAAGTTA GAGTTTATTC GTTGAATGAC GACTTGAAGC CAGCCAAAAG AGAAACTGTC
481      TTAACTTTCA TAGATCCTGA AGGATCAGAA GTTGACATGG TAGAAGAAAT TGATCATATT
541      GGAATTATCT CTTTTCCTGA CTTCAAGATT CCGTCTAATC CTAGATATGG TATGTGGACG
601      ATCAAGGCTA AATATAAAGA GGACTTTTCA ACAACTGGAA CCGCATATTT TGAAGTTAAA
661      GAATATGTCT TGCCACATTT TTCTGTCTCA ATCGAGCCAG AATATAATTT CATTGGTTAC
721      AAGAACTTTA AGAATTTTGA AATTACTATA AAAGCAAGAT ATTTTTATAA TAAAGTAGTC
781      ACTGAGGCTG ACGTTTATAT CACATTTGGA ATAAGAGAAG ACTTAAAAGA TGATCAAAAA
841      GAAATGATGC AAACAGCAAT GCAAAACACA ATGTTGATAA ATGGAATTGC TCAAGTCACA
901      TTTGATTCTG AAACAGCAGT CAAAGAACTG TCATACTACA GTTTAGAAGA TTTAAACAAC
961      AAGTACCTTT ATATTGCTGT AACAGTCATA GAGTCTACAG GTGGATTTTC TGAAGAGGCA
1021     GAAATACCTG GCATCAAATA TGTCCTCTCT CCCTACAAAC TGAATTTGGT TGCTACTCCT
1081     CTTTTCCTGA AGCCTGGGAT TCCATATCCC ATCAAGGTGC AGGTTAAAGA TTCGCTTGAC
1141     CAGTTGGTAG GAGGAGTCCC AGTAACACTG AATGCACAAA CAATTGATGT AAACCAAGAG
1201     ACATCTGACT TGGATCCAAG CAAAAGTGTA ACACGTGTTG ATGATGGAGT AGCTTCCTTT
1261     GTGCTTAATC TCCCATCTGG AGTGACGGTG CTGGAGTTTA ATGTCAAAAC TGATGCTCCA
1321     GATCTTCCAG AAGAAAATCA GGCCAGGGAA GGTTACCGAG CAATAGCATA CTCATCTCTC
1381     AGCCAAAGTT ACCTTTATAT TGATTGGACT GATAACCATA AGGCTTTGCT AGTGGGAGAA
1441     CATCTGAATA TTATTGTTAC CCCCAAAAGC CCATATATTG ACAAAATAAC TCACTATAAT
1501     TACTTGATTT TATCCAAGGG CAAAATTATC CACTTTGGCA CGAGGGAGAA ATTTTCAGAT
1561     GCATCTTATC AAAGTATAAA CATTCCAGTA ACACAGAACA TGGTTCCTTC ATCCCGACTT
1621     CTGGTCTATT ACATCGTCAC AGGAGAACAG ACAGCAGAAT TAGTGTCTGA TTCAGTCTGG
1681     TTAAATATTG AAGAAAAATG TGGCAACCAG CTCCAGGTTC ATCTGTCTCC TGATGCAGAT
1741     GCATATTCTC CAGGCCAAAC TGTGTCTCTT AATATGGCAA CTGGAATGGA TTCCTGGGTG
1801     GCATTAGCAG CAGTGGACAG TGCTGTGTAT GGAGTCCAAA GAGGAGCCAA AAAGCCCTTG
1861     GAAAGAGTAT TTCAATTCTT AGAGAAGAGT GATCTGGGCT GTGGGGCAGG TGGTGGCCTC
1921     AACAATGCCA ATGTGTTCCA CCTAGCTGGA CTTACCTTCC TCACTAATGC AAATGCAGAT
1981     GACTCCCAAG AAAATGATGA ACCTTGTAAA GAAATTCTCA GGCCAAGAAG AACGCTGCAA
2041     AAGAAGATAG AAGAAATAGC TGCTAAATAT AAACATTCAG TAGTGAAGAA ATGTTGTTAC
2101     GATGGAGCCT GCGTTAATAA TGATGAAACC TGTGAGCAGC GAGCTGCACG GATTAGTTTA
2161     GGGCCAAGAT GCATCAAAGC TTTCACTGAA TGTTGTGTCG TCGCAAGCCA GCTCCGTGCT
2221     AATATCTCTC ATAAAGACAT GCAATTGGGA AGGCTACACA TGAAGACCCT GTTACCAGTA
2281     AGCAAGCCAG AAATTCGGAG TTATTTTCCA GAAAGCTGGT TGTGGGAAGT TCATCTTGTT
2341     CCCAGAAGAA AACAGTTGCA GTTTGCCCTA CCTGATTCTC TAACCACCTG GGAAATTCAA
2401     GGCGTTGGCA TTTCAAACAC TGGTATATGT GTTGCTGATA CTGTCAAGGC AAAGGTGTTC
2461     AAAGATGTCT TCCTGGAAAT GAATATACCA TATTCTGTTG TACGAGGAGA ACAGATCCAA
2521     TTGAAAGGAA CTGTTTACAA CTATAGGACT TCTGGGATGC AGTTCTGTGT TAAAATGTCT
2581     GCTGTGGAGG GAATCTGCAC TTCGGAAAGC CCAGTCATTG ATCATCAGGG CACAAAGTCC
2641     TCCAAATGTG TGCGCCAGAA AGTAGAGGGC TCCTCCAGTC ACTTGGTGAC ATTCACTGTG
2701     CTTCCTCTGG AAATTGGCCT TCACAACATC AATTTTTCAC TGGAGACTTG GTTTGGAAAA
2761     GAAATCTTAG TAAAAACATT ACGAGTGGTG CCAGAAGGTG TCAAAAGGGA AAGCTATTCT
2821     GGTGTTACTT TGGATCCTAG GGGTATTTAT GGTACCATTA GCAGACGAAA GGAGTTCCCA
2881     TACAGGATAC CCTTAGATTT GGTCCCCAAA ACAGAAATCA AAAGGATTTT GAGTGTAAAA
2941     GGACTGCTTG TAGGTGAGAT CTTGTCTGCA GTTCTAAGTC AGGAAGGCAT CAATATCCTA
3001     ACCCACCTCC CCAAAGGGAG TGCAGAGGCG GAGCTGATGA GCGTTGTCCC AGTATTCTAT
3061     GTTTTTCACT ACCTGGAAAC AGGAAATCAT TGGAACATTT TTCATTCTGA CCCATTAATT
3121     GAAAAGCAGA AACTGAAGAA AAAATTAAAA GAAGGGATGT TGAGCATTAT GTCCTACAGA
3181     AATGCTGACT ACTCTTACAG TGTGTGGAAG GGTGGAAGTG CTAGCACTTG GTTAACAGCT
3241     TTTGCTTTAA GAGTACTTGG ACAAGTAAAT AAATACGTAG AGCAGAACCA AAATTCAATT
3301     TGTAATTCTT TATTGTGGCT AGTTGAGAAT TATCAATTAG ATAATGGATC TTTCAAGGAA
3361     AATTCACAGT ATCAACCAAT AAAATTACAG GGTACCTTGC CTGTTGAAGC CCGAGAGAAC
3421     AGCTTATATC TTACAGCCTT TACTGTGATT GGAATTAGAA AGGCTTTCGA TATATGCCCC
3481     CTGGTGAAAA TCGACACAGC TCTAATTAAA GCTGACAACT TTCTGCTTGA AAATACACTG
3541     CCAGCCCAGA GCACCTTTAC ATTGGCCATT TCTGCGTATG CTCTTTCCCT GGGAGATAAA
3601     ACTCACCCAC AGTTTCGTTC AATTGTTTCA GCTTTGAAGA GAGAAGCTTT GGTTAAAGGT
3661     AATCCACCCA TTTATCGTTT TTGGAAAGAC AATCTTCAGC ATAAAGACAG CTCTGTACCT
3721     AACACTGGTA CGGCACGTAT GGTAGAAACA ACTGCCTATG CTTTACTCAC CAGTCTGAAC
3781     TTGAAAGATA TAAATTATGT TAACCCAGTC ATCAAATGGC TATCAGAAGA GCAGAGGTAT
3841     GGAGGTGGCT TTTATTCAAC CCAGGACACA ATCAATGCCA TTGAGGGCCT GACGGAATAT
3901     TCACTCCTGG TTAAACAACT CCGCTTGAGT ATGGACATCG ATGTTTCTTA CAAGCATAAA
3961     GGTGCCTTAC ATAATTATAA AATGACAGAC AAGAATTTCC TTGGGAGGCC AGTAGAGGTG
4021     CTTCTCAATG ATGACCTCAT TGTCAGTACA GGATTTGGCA GTGGCTTGGC TACAGTACAT
4081     GTAACAACTG TAGTTCACAA AACCAGTACC TCTGAGGAAG TTTGCAGCTT TTATTTGAAA
4141     ATCGATACTC AGGATATTGA AGCATCCCAC TACAGAGGCT ACGGAAACTC TGATTACAAA
4201     CGCATAGTAG CATGTGCCAG CTACAAGCCC AGCAGGGAAG AATCATCATC TGGATCCTCT
4261     CATGCAGTGA TGGACATCTC CTTGCCTACT GGAATCAGTG CAAATGAAGA AGACTTAAAA
4321     GCCCTTGTGG AAGGGGTGGA TCAACTATTC ACTGATTACC AAATCAAAGA TGGACATGTT
4381     ATTCTGCAAC TGAATTCGAT TCCCTCCAGT GATTTCCTTT GTGTACGATT CCGGATATTT
4441     GAACTCTTTG AAGTTGGGTT TCTCAGTCCT GCCACTTTCA CAGTGTACGA ATACCACAGA
4501     CCAGATAAAC AGTGTACCAT GTTTTATAGC ACTTCCAATA TCAAAATTCA GAAAGTCTGT
4561     GAAGGAGCCG CGTGCAAGTG TGTAGAAGCT GATTGTGGGC AAATGCAGGA AGAATTGGAT
4621     CTGACAATCT CTGCAGAGAC AAGAAAACAA ACAGCATGTA AACCAGAGAT TGCATATGCT
4681     TATAAAGTTA GCATCACATC CATCACTGTA GAAAATGTTT TTGTCAAGTA CAAGGCAACC
4741     CTTCTGGATA TCTACAAAAC TGGGGAAGCT GTTGCTGAGA AAGACTCTGA GATTACCTTC
4801     ATTAAAAAGG TAACCTGTAC TAACGCTGAG CTGGTAAAAG GAAGACAGTA CTTAATTATG
4861     GGTAAAGAAG CCCTCCAGAT AAAATACAAT TTCAGTTTCA GGTACATCTA CCCTTTAGAT
4921     TCCTTGACCT GGATTGAATA CTGGCCTAGA GACACAACAT GTTCATCGTG TCAAGCATTT
4981     TTAGCTAATT TAGATGAATT TGCCGAAGAT ATCTTTTTAA ATGGATGCTA A

Quality Control:cDNA sequencing results is available upon request.