CFH / Complement Factor H Protein (His Tag)

Complement Factor H / CFH Protein

Complement Factor H / CFH Protein Product Information

• Synonym: DAAP-278B20.1, CAK, CD167, DDR, EDDR1, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE
• Protein Construction: A DNA sequence encoding the C-terminal segment of CFH isoform a ( NP_000177.2 ), corresponding to amino acid ( Ser 860 - Arg 1231 ) was expressed, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus
• Source: Human
• Expression Host: Human Cells

Complement Factor H / CFH Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to bind biotinylated human DMP1 in a functional ELISA
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 860
• Molecular Mass: The recombinant human CFH consists of 383 amino acids and has a calculated molecular mass of 43 kDa. The apparent molecular mass of rh CFH is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CFH protein

Complement Factor H / CFH Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Complement Factor H / CFH Protein Description

Complement factor H, also known as H factor 1, and CFH, is a secreted protein which contains 20 Sushi ( CCP / SCR ) domains. It is a large soluble glycoprotein that circulates in human plasma (at a concentration of 500–800 micrograms per milliliter). The main job of CFH is to regulate the Alternative Pathway of the complement system, ensuring that the complement system is directed towards pathogens and does not damage host tissue. CFH regulates complement activation on self cells by possessing both cofactor activity for the Factor I mediated C3b cleavage, and decay accelerating activity against the alternative pathway C3 convertase, C3bBb. CFH protects self cells from complement activation but not bacteria/viruses. Due to the central role that CFH plays in the regulation of complement, there are many clinical implications arrising from aberrant CFH activity. Defects in CFH are the cause of complement factor H deficiency (CFH deficiency) and hemolytic-uremic syndrome (HUS). Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4) which is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world.

References

1. Schulz TF. et al., 1986, Eur. J. Immunol. 16:1351-5.
2. Buddles MR.H. et al., 2000, Am. J. Hum. Genet. 66:1721-2.
3. Hageman GS. et al., 2005, Proc. Natl. Acad. Sci. USA. 102:7227-32.
4. Liu T. et al., 2005, J. Proteome Res. 4: 2070-80.
5. Gregory SG. et al., 2006, Nature. 441: 315-21.
6. Chen R. et al., 2009, J. Proteome Res. 8: 651-61.