Anti-C5a Antibody

Cat: 10604-MM04
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Anti-C5a Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-C5a Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human C5a
No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2 Human alpha-2-macroglobulin / A2M E.coli cell lysate
Immunogen
Recombinant Human Complement C5a Protein (Catalog#10604-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Complement C5a (rh Complement C5a; Catalog#10604-HNAE; Leu 679-Arg 751). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #5E 1D1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-C5a Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:500-1:1000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-C5a Antibody Alternative Names
Anti-C5a Antibody;Anti-C5b Antibody;Anti-C5D Antibody;Anti-C5Da Antibody;Anti-Complement 5a Antibody;Anti-CPAMD4 Antibody;Anti-ECLZB Antibody
C5a Background Information

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.

Full Name
complement component 5
References
  • Guo RF, et al.. (2005) Role of C5a in inflammatory responses. Annu Rev Immunol. 23: 821-52.
  • Guo RF, et al. (2006) C5a, a therapeutic target in sepsis. Recent Pat Antiinfect Drug Discov. 1(1): 57-65.
  • Manthey HD, et al. (2009) Complement component 5a (C5a). Int J Biochem Cell Biol. 41(11): 2114-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-C5a Antibody Rabbit Polyclonal ELISA 10604-T16
    Anti-C5a Antibody Rabbit Monoclonal ELISA 10604-R442
    Anti-C5a Antibody Mouse Monoclonal ELISA(Cap) 10604-MM06
    Anti-C5a Antibody Rabbit Monoclonal IHC-P 10604-R215
    Anti-C5a Antibody Rabbit Monoclonal ELISA 10604-R241
    Anti-C5a Antibody Rabbit Polyclonal ELISA 10604-RP02
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