C2 / Complement Component 2 Protein

Complement Component C2 Protein Datasheet

C2 Protein Product Information

• Synonym: C2, CO2, DKFZp779M0311
• Protein Construction: A DNA sequence encoding the human complement component 2 (C2) precursor (NP_000054.2) (Met 1-Leu 752) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

C2 Protein QC Testing

• Purity: >95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 21
• Molecular Mass: The recombinant human complement component C2/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and has a calculated molecular mass of 110 KDa. As a result of glycosylation, the apparent molecular mass of rhC2/Fc monomer is approximately 110-130 KDa in reduced SDS-PAGE
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

C2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃ after receipt. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

C2 Protein Description

Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.

References

1. Bentley, DR. et al., 1984, Proc. Natl. Acad. Sci . 81:1212-1215.
2. Akama, H. et al., 1995, J. Biol. Chem. 270: 2674-2678.
3. Wallis, R. et al., 2007, J. Biol. Chem. 282: 7844-7851.
4. Wang, X.. et al., 1998, Immunology. 93: 184-191.
5. Alper, C.A. et al., 2003, J. Clin. Immunol. 23: 297-305.