C2 / Complement Component 2 Antibody

Complement Component C2 / C2 Antibody Datasheet

Complement Component C2 / C2 Antibody Product Information

• Immunogen: Recombinant human C2 protein ( Catalog#10154-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 002
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human complement component 2 ( rh C2 ; Catalog#10154-H08H ; Met 1 - Leu 752 ; NP_000054.2 ).

Complement Component C2 / C2 Antibody Usage Guide

• Specificity: Human Complement Component 2
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human C2 in WB. Using a DAB detection system, the detection limit for human C2 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human C2. The detection limit for human C2 is 0.0049 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Complement Component C2 / C2 Antibody Background

Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.

References

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2. Akama, H. et al., 1995, J. Biol. Chem. 270: 2674-2678.
3. Wallis, R. et al., 2007, J. Biol. Chem. 282: 7844-7851.
4. Wang, X.. et al., 1998, Immunology. 93: 184-191.
5. Alper, C.A. et al., 2003, J. Clin. Immunol. 23: 297-305.