Mouse C1s Gene cDNA Clone / ORF Clone

C1S cDNA Clone Datasheet

C1S cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001097617.1
• cDNA Size: 2085
• Gene Synonym: C1sa; AA959438; AI255193; AI327365; MGC37773; MGC132830; C1s
• Source: Mouse

C1S cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus complement component 1, s subcomponent DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for eight point mutations: 245 A/G resulting in the amino acid Asp substitution by Gly, 275 G/A resulting in the amino acid Arg substitution by Lys, 931 C/G resulting in the amino acid Gln substitution by Glu, 99 T/C, 174 T/C, 675 G/A, 699 C/T, 2010 C/T not causing the amino acid variation.

C1S cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

C1S cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

C1S cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCAAAT CGCCAGGGAT GTGGTGTTTG GTCCTGTTTT CTCTTTTGGC ATCATTTTCT
61       GCTGAGCCCA CCATGCATGG GGAGATCCTG TCCCCTAACT ATCCCCAGGC GTACCCCAAT
121      GACGTCGTGA AATCTTGGGA CATTGAAGTC CCAGAGGGGT TTGGGATTCA CCTCTACTTT
181      ACCCATGTGG ACATAGAGCC GTCAGAGAGC TGTGCATACG ACTCAGTGCA GATAATCTCA
241      GGAGGCATCG AGGAAGGGAG ACTCTGTGGC CAGAAGACCA GCAAGAGCCC CAACTCCCCC
301      ATTATAGAAG AGTTTCAGTT CCCATACAAT AAACTTCAGG TGGTCTTTAC CTCAGACTTC
361      TCCAACGAAG AACGGTTTAC GGGCTTTGCA GCATACTACA CTGCCATAGA CATAAATGAA
421      TGCACAGATT TTACAGATGT CCCCTGTAGC CACTTCTGCA ATAACTTCAT TGGTGGATAC
481      TTCTGCTCCT GTCCCCCAGA ATACTTCCTC CACGATGACA TGAGGAATTG TGGAGTCAAC
541      TGTAGTGGAG ATGTGTTCAC TGCCCTGATT GGGGAGATCT CAAGTCCCAA TTATCCCAAT
601      CCATACCCGG AGAACTCAAG GTGTGAATAC CAGATTCAGC TGCAGGAGGG CTTCCAAGTG
661      GTGGTGACGA TGCAAAGAGA AGATTTTGAT GTGGAGCCTG CTGACTCAGA GGGGAACTGC
721      CCTGACAGTT TAACTTTTGC TTCAAAAAAC CAACAGTTTG GTCCTTACTG TGGTAATGGG
781      TTCCCTGGAC CGCTAACTAT TAGAACCCAG AGCAATACTC TCGGCATTGT CTTTCAAACT
841      GACCTAATGG GGCAAAAGAA GGGTTGGAAG CTTCGTTACC ATGGAGATCC CATCTCCTGT
901      GCCAAAAAAA TCACTGCCAA TTCTACTTGG GAGCCCGATA AGGCAAAATA CGTGTTTAAA
961      GATGTGGTGA AGATAACCTG TGTGGACGGA TTTGAAGTTG TGGAGGGACA TGTTAGCTCT
1021     ACTTCATACT ATTCCACTTG TCAAAGCGAT GGACAGTGGA GCAACTCCGG TCTAAAATGT
1081     CAACCTGTGT ATTGTGGAAT TCCAGATCCC ATTGCGAATG GTAAAGTTGA AGAGCCAGAG
1141     AATAGTGTGT TTGGTACTGT TGTTCACTAC ACGTGTGAAG AGCCATATTA CTACATGGAA
1201     CATGAAGAAG GTGGGGAGTA TCGATGTGCT GCAAATGGGA GATGGGTAAA TGACCAGCTG
1261     GGTATTGAGC TTCCAAGATG TATTCCAGCC TGTGGAGTAC CCACCGAGCC CTTTCAAGTG
1321     CATCAGAGGA TATTTGGAGG ACAACCTGCA AAGATTGAAA ATTTTCCCTG GCAAGTCTTC
1381     TTTAATCACC CCAGAGCTAG TGGGGCTCTT ATTAATGAGT ACTGGGTGCT GACGGCTGCT
1441     CACGTTTTGG AGAAAATCTC CGACCCTTTA ATGTATGTCG GGACCATGTC TGTGAGGACA
1501     ACTCTCTTGG AAAATGCCCA GAGACTCTAC AGTAAACGTG TCTTTATTCA TCCCAGCTGG
1561     AAGAAAGAAG ATGACCCAAA CACACGGACA AATTTTGACA ATGACATTGC CCTGGTGCAG
1621     CTGAAAGACC CTGTGAAAAT GGGACCCAAA GTCTCCCCCA TCTGCCTACC AGGCACCTCC
1681     TCAGAGTACA ACGTCTCACC GGGTGACATG GGGCTGATCT CAGGGTGGGG CAGTACAGAA
1741     AAGAAAGTCT TTGTTATCAA CCTCAGAGGG GCAAAGGTAC CAGTAACCTC TTTAGAAACC
1801     TGCAAACAGG TGAAAGAGGA GAACCCCACT GTAAGGCCTG AGGACTATGT TTTCACTGAC
1861     AACATGATCT GTGCTGGCGA GAAAGGTGTT GACAGCTGTC ACGGGGACAG CGGCGGGGCT
1921     TTTGCTTTCC AGGTCCCCAA TGTCACGGTC CCCAAATTCT ATGTAGCTGG CTTGGTGTCT
1981     TGGGGGAAAA GGTGTGGGAC TTATGGGGTT TACACAAAGG TAAAGAATTA CGTGGACTGG
2041     ATCCTGAAAA CTATGCAGGA GAATAGTGGG CCCAGGAAGG ACTAA

 

Quality Control:cDNA sequencing results is available upon request.