Complement C1s Antibody

Complement C1s Antibody Datasheet

Complement C1s Antibody Product Information

• Immunogen: Recombinant Human Complement C1s protein (Catalog#10220-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 006
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Complement C1s (rh Complement C1s; Catalog#10220-H08H; NP_001725.1; Met 1-Asp 688).

Complement C1s Antibody Usage Guide

• Specificity: Human Complement C1s
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human C1S. The detection limit for Human C1S is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Complement C1s Antibody Background

Complement is an integral component of the adaptive and innate immune systems and represents one of the major effector systems for the immune responses. The classical complement pathway is triggered by C1, a complex composed of the binding protein C1q and two proenzymes, C1r and C1s. Upon binding of IgG to the head of C1q, C1r undergoes autoactivation and in turn cleaves and activates C1s. As a highly specific serine protease, C1s executes the catalytic function of the C1 complex: the cleavage of C4 and C2, and thus instigates a sequence of activation steps of other components of the complement system, culminating in the formation of the membrane attack complex which induces cell lysis. The single-chain proenzyme C1s is processed into the active form of disulfide-linked two chains: the A chain contains multiple domains in the order of CUB1-EGF-CUB2-CCP1 -CCP2-Activation Peptide, while the B chain contains the serine protease catalytic domain. The only other protein known to interact with C1s physiologically is SerpinC1, an inhibitor of serine protease, which inhibits C1s activity and thus plays a regulatory role in controlling the function of C1s enzyme.

References

1. Illy, C. et al., 1991. Biochemistry. 30: 7135-7141.
2. Kusumoto, H. et al., 1988, Proc. Natl. Acad. Sci. USA 85:7307-7311.
3. Kerr, F.K. et al., 2005, J.Biol. Chem. 280: 39510-39514.
4. Busby Jr, W. H. et al., 2000, J. Biol. Chem. 275:37638-37644.
5. Gal, P. et al., 2002, Immunobiology. 205: 383-394.