Mouse COCH Gene cDNA Clone / ORF Clone

COCH cDNA Clone Datasheet

COCH cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007728.5
• cDNA Size: 1659
• Gene Synonym: AW122937; Coch-5B2; D12H14S564E; Coch
• Source: Mouse

COCH cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus coagulation factor C homolog (Limulus polyphemus) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 1065 C/T not causing the amino acid variation.

COCH cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

COCH cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

COCH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG
61       CCCCGGTTCG CGCGCGCCGA GGGAGCGGTT CCCATTCCTG TCACCTGCTT TACCAGAGGC
121      CTGGATATCC GAAAAGAGAA AGCAGATGTT CTCTGCCCAG GAGGCTGCTC TCTTGAGGAA
181      TTCTCTGTGT TTGGGAACAT AGTGTATGCG TCAGTGTCCA GCATCTGCGG CGCTGCTGTC
241      CATAGGGGAG TGATTGGCAC CTCAGGGGGA CCTGTGCGTG TCTACAGCCT TCCTGGTCGA
301      GAGAACTACT CCTCGGTAGA TGCCAACGGC ATCCAGTCTC AGATGCTTTC CCGATGGTCC
361      GCGTCCTTCG CTGTGACCAA AGGCAAAAGC AGTACCCAGG AAGCCACAGG ACGGGCAGTG
421      TCCACAGCCC ACCCACCTTC AGGTAAAAGA CTAAAGAAGA CACCAGAGAA GAAGACTGGC
481      AACAAAGACT GTAAGGCAGA CATTGCATTT CTCATTGATG GAAGCTTCAA TATTGGGCAG
541      CGCCGATTTA ATTTGCAGAA GAATTTTGTT GGGAAAGTGG CACTAATGTT GGGAATTGGA
601      ACAGAAGGAC CACACGTGGG TCTCGTTCAA GCCAGTGAAC ACCCCAAAAT AGAATTTTAC
661      TTGAAAAACT TTACTTCAGC CAAAGATGTC TTGTTTGCCA TAAAAGAAGT AGGTTTCCGA
721      GGGGGTAACT CCAACACAGG AAAAGCCTTG AAGCACACTG CTCAGAAATT CTTTACAGCA
781      GACACTGGTG TGAGAAAAGG AATACCAAAA GTGGTGGTAG TGTTTATTGA TGGTTGGCCC
841      TCTGATGACA TTGAGGAAGC AGGCATTGTG GCCAGAGAGT TTGGTGTCAA TGTATTTATA
901      GTTTCTGTGG CCAAGCCCAT TCCTGAAGAA CTGGGGATGG TTCAAGATGT TGCATTTGTT
961      GACAAGGCTG TGTGTCGGAA TAATGGCTTC TTCTCTTATC ACATGCCCAA CTGGTTTGGC
1021     ACTACAAAAT ATGTGAAGCC TCTGGTGCAG AAGCTCTGTA CGCATGAACA GATGATGTGC
1081     AGCAAAACCT GCTACAACTC AGTGAACATT GCCTTTCTGA TTGACGGCTC CAGCAGTGTT
1141     GGAGATAGCA ATTTCCGCCT CATGCTAGAA TTTGTTTCTA ACATAGCGAA GACATTTGAA
1201     ATCTCAGACA TTGGAGCCAA GATAGCTGCT GTACAGTTCA CTTATGACCA GCGCACCGAG
1261     TTCAGTTTCA CTGACTATAA TACCAAAGAG AACGTCCTAG CTGTCCTAGC GAACATCCGC
1321     TACATGAGTG GTGGCACAGC TACTGGTGAT GCCATCGCCT TTACTGTTAG AAATGTATTT
1381     GGTCCCATAA GGGACAGCCC CAACAAAAAC TTCCTGGTTA TTGTCACAGA TGGGCAGTCC
1441     TATGATGATG TCCGAGGCCC TGCTGCAGCT GCCCATGATG CAGGTATCAC CATCTTCTCT
1501     GTTGGTGTGG CTTGGGCACC GCTGGATGAC CTGAGAGATA TGGCCTCTAA ACCCAAAGAG
1561     TCACACGCTT TCTTTACCAG AGAGTTCACA GGGTTAGAAC CAATTGTCTC TGACGTCATC
1621     AGAGGCATTT GTAGAGACTT CTTAGAATCC CAGCAATAA

 

Quality Control:cDNA sequencing results is available upon request.