Coagulation Factor VIII / FVIII / F8 (Heavy Chain) Antibody

Coagulation Factor VIII / FVIII / F8 Antibody Datasheet

Coagulation Factor VIII / FVIII / F8 Antibody Product Information

• Immunogen: Recombinant Human Coagulation Factor VIII / FVIII / F8 protein
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 226
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Coagulation Factor VIII / FVIII / F8 protein.

Coagulation Factor VIII / FVIII / F8 Antibody Usage Guide

• Specificity: Human Coagulation Factor VIII / FVIII / F8 Heavy Chain
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human FⅧ in WB.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FⅧ. The detection limit for Human FⅧ is approximately 0.078ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Coagulation Factor VIII / FVIII / F8 Antibody Background

Coagulation Factor VIII, also known as FVIII and F8, is a member of the multicopper oxidase family. Coagulation Factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. It contains 3 F5/8 type A domains, 2 F5/8 type C domains and 6 plastocyanin-like domains. FVIII is synthesized in the liver, and perhaps in other tissues. It is a coagulation cofactor which circulates bound to von Willebrand factor and is part of the intrinsic coagulation pathway. It is a macromolecular complex composed of two separate entities, one of which, when deficient, results in hemophilia A, and the other, when deficient, results in von Willebrand's disease. Hemophilia A is a disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery.

References

1. Toole JJ. et al., 1984, Nature. 312(5992): 342-7.
2. Truett MA. et al., 1985, DNA. 4(5): 333-49.
3. Antonarakis SE. et al., 1995, Haemost. 74(1): 322-8.