Coagulation Factor IX / F9 Antibody (Antigen Affinity Purified)

Coagulation Factor IX / F9 Antibody ( Antigen Affinity Purified ) Datasheet

Coagulation Factor IX / F9 Antibody Product Information

• Immunogen: Recombinant Human Coagulation Factor IX / F9 protein (Catalog#11503-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Coagulation Factor IX / F9 (rh Coagulation Factor IX / F9; Catalog#11503-H08H; AAB59620.1; Met 1-Thr 461). Coagulation Factor IX / F9 specific IgG was purified by Human Coagulation Factor IX / F9 affinity chromatography.

Coagulation Factor IX / F9 Antibody Usage Guide

• Specificity: Human Coagulation Factor IX / F9
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human F9 in WB. Using a DAB detection system, the detection limit for Human F9 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human F9. The detection limit for Human F9 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Coagulation Factor IX / F9 Antibody Background

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to thepeptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ions, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis.Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B (HEMB) which also known as Christmas disease.

References

1. Onay U.V., et al., 2003, Br. J. Haematol. 120: 656-659.
2. Vidal F., et al., 2000, Br. J. Haematol. 111: 549-551.
3. Simioni P., et al., 2009, N. Engl. J. Med. 361: 1671-1675.
4. Espinos C., et al., 2009, Haematologica 88: 235-236.