Anti-Factor IX Antibody

Cat: 11503-RP02
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Anti-Factor IX Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Factor IX Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human Factor IX
Immunogen
Recombinant Human Coagulation Factor IX / F9 protein (Catalog#11503-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Coagulation Factor IX / F9 (rh Coagulation Factor IX / F9; Catalog#11503-H08H; AAB59620.1; Met 1-Thr 461). Coagulation Factor IX / F9 specific IgG was purified by Human Coagulation Factor IX / F9 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Factor IX Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Factor IX Antibody (Rabbit Polyclonal antibody) Images
Human Coagulation Factor IX / FIX / F9 Western blot (WB) 13408

Anti-F9 rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:55 kDa

Anti-Factor IX Antibody Alternative Names
Anti-Coagulation factor 9 Antibody;Anti-Coagulation factor IX Antibody;Anti-FIX Antibody;Anti-HEMB Antibody;Anti-P19 Antibody;Anti-PTC Antibody;Anti-THPH8 Antibody
Factor IX Background Information

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.

Full Name
coagulation factor IX
References
  • Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659.
  • Vidal F., et al., 2000, Br. J. Haematol. 111:549-551.
  • Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675.
  • Espinos C., et al., 2009, Haematologica 88:235-236.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Factor IX Antibody Rabbit Polyclonal ELISA 50362-RP01
    Anti-Factor IX Antibody Rabbit Polyclonal ELISA 50362-T16
    Anti-Factor IX Antibody Rabbit Monoclonal ELISA 50362-R003
    Anti-Factor IX Antibody Rabbit Monoclonal ELISA(Cap) 11503-R044
    Anti-Factor IX Antibody Rabbit Monoclonal ELISA(Det) 11503-R014
    Anti-Factor IX Antibody Rabbit Monoclonal WB,ELISA 11503-R034
    Anti-Factor IX Antibody Rabbit Polyclonal ELISA 11503-RP01
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