Anti-Clusterin Antibody

Cat: 11297-RP02
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Anti-Clusterin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Clusterin Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human Clusterin
Immunogen
Recombinant Human Clusterin / Apolipoprotein J / Apo-J protein (Catalog#11297-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). Clusterin specific IgG was purified by Human Clusterin affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Clusterin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Clusterin Antibody (Rabbit Polyclonal antibody) Images
Human Clusterin/Apolipoprotein J/Apo-J Immunohistochemistry(IHC) 4391
Immunochemical staining of human CLU in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Human Clusterin/Apolipoprotein J/Apo-J Immunoprecipitation(IP) 8080

CLU was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-CLU rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CLU rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 47 kDa

Observed band size: 47 kDa

Human Clusterin/Apolipoprotein J/Apo-J Western blot (WB) 13388

Anti-CLU rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:57 kDa

Anti-Clusterin Antibody Alternative Names
Anti-AAG4 Antibody;Anti-APO-J Antibody;Anti-APOJ Antibody;Anti-CLI Antibody;Anti-CLU1 Antibody;Anti-CLU2 Antibody;Anti-KUB1 Antibody;Anti-NA1/NA2 Antibody;Anti-SGP-2 Antibody;Anti-SGP2 Antibody;Anti-SP-40 Antibody;Anti-TRPM-2 Antibody;Anti-TRPM2 Antibody
Clusterin Background Information

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

Full Name
clusterin
References
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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    Anti-Clusterin Antibody (FITC) Mouse Monoclonal FCM 11297-MM01-F
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    Anti-Clusterin Antibody Mouse Monoclonal WB,ELISA,FCM,ICC/IF,IF 11297-MM01
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    Anti-Clusterin Antibody Rabbit Polyclonal ELISA 11297-T16
    Anti-Clusterin Antibody Rabbit Monoclonal ELISA 11297-R012
    Anti-Clusterin Antibody Rabbit Polyclonal IP 11297-T04
    Anti-Clusterin Antibody Rabbit Polyclonal WB,ICC/IF,IF 11297-T34
    Anti-Clusterin Antibody (PE) Mouse Monoclonal FCM 11297-MM01-P
    Anti-Clusterin Antibody Rabbit Monoclonal FCM,ICC/IF,IF 11297-R013
    Anti-Clusterin Antibody (FITC) Rabbit Monoclonal FCM 11297-R013-F
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