|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CLU was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate0.5 µL anti-CLU rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CLU rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 47 kDa
Observed band size: 47 kDa
Anti-CLU rabbit polyclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell Lysate
Lane B: Hela Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:52 kDa
Observed band size:57 kDa
Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Clusterin Antibody (FITC)||Mouse||Monoclonal||FCM||11297-MM01-F|
|Anti-Clusterin Antibody (PE)||Mouse||Monoclonal||FCM||11297-MM01-P|
|Anti-Clusterin Antibody (FITC)||Rabbit||Monoclonal||FCM||11297-R013-F|