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Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CLU Antibody Product Information
Immunogen:Recombinant Human Clusterin / Apolipoprotein J / Apo-J protein (Catalog#11297-H08H)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). Clusterin specific IgG was purified by Human Clusterin affinity chromatography.
Human CLU Antibody Usage Guide
Specificity:Human Clusterin / Apolipoprotein J / Apo-J
Application:WB, ELISA, IHC-P, IP

WB: 10-20 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CLU. The detection limit for Human CLU is approximately 0.00975 ng/well.

IHC-P: 0.1-2 μg/m

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CLU Antibody WB Application Image
Human CLU Antibody IHC Application Image
Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Immunochemical staining of human CLU in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Human CLU Antibody IP Application Image
Other CLU Antibody Products
Clusterin/Apolipoprotein J/Apo-J Background

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

Human Clusterin/Apolipoprotein J/Apo-J References
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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    Catalog: 11297-RP02-50
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