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Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Mouse MAb

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Human CLU Antibody Product Information
Immunogen:Recombinant Human Clusterin / Apolipoprotein J / Apo-J Protein (Catalog#11297-H08H)
Clone ID:9E2E2
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CLU Antibody Usage Guide
Specificity:Human Clusterin / Apolipoprotein J / Apo-J
No cross-reactivity in ELISA with
Mouse CLU / Clusterin / Apolipoprotein J / Apo-J
Human cell lysate (293 cell line)
Application:WB, ELISA, ICC/IF, IF, FCM

WB: 10-30 μg/ml

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CLU. The detection limit for Human CLU is approximately 0.16 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CLU Antibody WB Application Image
Human CLU Antibody IF Application Image
Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Mouse MAb, Immunofluorescence
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Immunofluorescence staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CLU monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Human CLU Antibody FC Application Image
Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Mouse MAb
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Flow cytometric analysis of Human CLU expression on A549 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other CLU Antibody Products
Clusterin/Apolipoprotein J/Apo-J Background

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

Human Clusterin/Apolipoprotein J/Apo-J References
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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    Catalog: 11297-MM01-100
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