Chromatin Remodeling

Chromatin remodeling involves the effective shifting of nucleosome cores, which are composed of histone octamers, that consist of a H3(2):H4(2) tetramer and two dimers of H2A and H2B, along the length of the DNA molecule, a process known as "nucleosome sliding". Recent studies suggest that this shift may involve the actual disassembly and reassembly of the nucleosome core.

Sino Biological offers a comprehensive set of tools for Chromatin Remodeling related studies, including recombinant proteins, antibodies (rabbit mAbs, mouse mAbs, rabbit pAbs), ELISA kits, and ORF cDNA clones.

Chromatin Remodeling Related Products

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Histone Acetylation

ING5

KAT8^*

Histone Deacetylation (HDAC)

HDAC1	HDAC10	SIRT4^*
HDAC2	HDAC11	SIRT5^*
HDAC3	SIRT1	SIRT6^*
HDAC4	SIRT2^*	SIRT7^*
HDAC8	SIRT3^*

Histone Methylation

DOT1L / KMT4	SETD7 / SET7/9	SUV39H2^*
EZH2^*	SETD8 / PR-Set7	SUV420H1^*
PRDM2 / RIZ1	SMYD2^*	SUV420H2
PRMT5 / SKB1	SUV39H1^*

Histone Demethylation

LSD1

ISWI

RBBP4 / RBAP48

Chromatin Remodeling Background

Chromatin remodeling is accomplished, at least in part, by ATPase containing complexes, referred to as the SWI / SNF family. Chromatin remodeling complexes are classed into four subfamilies based upon their associated ATPase. The four ATPases associated with chromatin remodeling complexes are SWI / SNF and Brg1; imitation switch (ISWI); Mi-2 (CHD1) and INO80.

The SWI / SNF complex is a 2 MDa multi-subunit DNA-dependent ATPase that contributes to the regulation of gene transcription by altering chromatin structure. Recent studies have revealed that the SWI / SNF complex is targeted to promoters via direct interactions with transcription activators and have provided insights into mechansims by which the complex alters nucleosome structure and contributes to the remodeling of chromatin.The ISWI complexes are divided into the nucleosome remodeling factor (NURF), chromatin accessibility complex (CHRAC), and ATP-dependent chromatin and remodeling factor (ACF) complexes. NURF is composed of ISWI and three additional subunits, NURF301, NURF55 and NURF38. The SWI/SNF family members differentiate at the level of DNA sequence and gene structure.

Chromatin Remodeling Related Studies

Wilson CJ, et al. (1996) RNA polymerase II holoenzyme contains SWI / SNF regulators involved in chromatin remodeling. Cell. 84(2): 235-44.
Peterson CL, et al. (2000) Promoter targeting and chromatin remodeling by the SWI / SNF complex. Curr Opin Genet Dev. 10(2): 187-92.
Xue Y, et al. (1998) NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities. Mol Cell. 2(6): 851-61.