Mouse CHRDL1 Gene cDNA Clone / ORF Clone

CHRDL1 cDNA Clone Datasheet

CHRDL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031258.3
• cDNA Size: 1002
• Gene Synonym: CHL; CHL1; VOPT; Nrln1; BB139951; Chrdl1
• Source: Mouse

CHRDL1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chordin-like 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 632 A/G resulting in the amino acid Asn substitution by Ser.

CHRDL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHRDL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHRDL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATGGCA TGAAATACAT CATTTCCTTA TTTTTCATCT TTGTTTTCCT AGAAGGAAGC
61       AAAACAGAAC AAGTAAAACA CTCAGACACA TATTGCGTGT TTCAAGACAA GAAGTATAGA
121      GTGGGTGAGA AATGGCATCC CTACCTGGAA CCGTATGGAC TGGTTTACTG TGTGAACTGC
181      ATCTGCTCTG AGAATGGGAA TGTGCTTTGC AGCCGAGTCA GATGTCCAAG TCTTCATTGC
241      CTTTCACCCG TGCATATTCC TCATCTCTGT TGCCCCCGCT GCCCAGACTC CTTACCACCA
301      GTGAACAATA AGGTGACCAG CAAGTCATGC GAATACAATG GAACCACTTA CCAACATGGA
361      GAACTGTTCA TAGCTGAAGG GCTCTTTCAG AACCGGCAAC CCAATCAGTG CAGTCAGTGT
421      AGCTGCTCGG AGGGGAATGT ATACTGTGGT CTCAAGACTT GCCCCAAACT GACCTGTGCA
481      TTCCCAGTCT CTGTTCCAGA TTCTTGCTGC CGAGTATGCA GAGGGGATGC AGAATTATCG
541      TGGGAACATG CGGATGGTGA TATCTTCCGG CAACCTGCCA ACAGAGAAGC AAGACATTCT
601      TACCTCCGTT CCCCCTACGA TCCTCCACCA AGCAGACAAG CTGGAGGTCT TCCCCGCTTT
661      CCTGGGAGCA GAAGTCACCG GGGAGCTGTT ATAGATTCCC AGCAAGCATC CGGGACCATC
721      GTGCAGATTG TCATCAATAA CAAGCACAAA CATGGACAAG TGTGTGTTTC CAATGGAAAG
781      ACCTACTCTC ATGGAGAGTC CTGGCACCCA AATCTACGAG CATTTGGCAT TGTGGAATGT
841      GTACTATGCA CTTGTAATGT CACCAAGCAA GAATGTAAGA AAATCCACTG CCCCAATCGA
901      TACCCCTGCA AGTATCCTCA AAAAATAGAT GGAAAGTGCT GCAAGGTGTG CCCAGGTAAA
961      AAGGCAAAAG GTGCATTGGC TGGAGGCCCT GCCTTTGGTT GA

 

Quality Control:cDNA sequencing results is available upon request.