Neuralin-1 / CHRDL1 Protein (His Tag)

Neuralin-1 / CHRDL1 (Chordin-Like Protein 1) Protein

Neuralin-1 / CHRDL1 Protein Product Information

• Synonym: CHRDL1 , RP6-141H5.1, CHL, NRLN1, VOPT, dA141H5.1
• Protein Construction: A DNA sequence encoding the human CHRDL1 (NP_660277.2) ( Met 1- Cys 456 ) with a C-terminal polyhistidine tag was expressed
• Source: Human
• Expression Host: Human Cells

Neuralin-1 / CHRDL1 Protein QC Testing

• Purity: > 75 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 28
• Molecular Mass: The secreted recombinant human CHRDL1 comprises 440 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, rh CHRDL1 migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: CHRDL1 protein

Neuralin-1 / CHRDL1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Neuralin-1 / CHRDL1 Protein Description

Chordin-like protein 1, also known as Neuralin-1, Ventroptin, NRLN1, and CHRDL1, is a secreted protein which contains three VWFC domains. CHRDL1 is an antagonist of bone morphogenetic protein 4 (BMP-4) which antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. It is mainly expressed in the ventral retina, not only with a ventral high-dorsal low gradient but also with a nasal high-temporal low gradient at later stages. Misexpression of CHRDL1 altered expression patterns of several topographic genes in the retina and projection of the retinal axons to the tectum along both axes. CHRDL1 alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. It contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. CHRDL1 may play a crucial role in dorsoventral axis formation. It may play a role in embyonic bone formation. CHRDL1 may also play an important role in regulating retinal angiogenesis trough modulation of BMP4 actions in endothelial cells.

References

1. Sakuta, H. et al., 2001, Science. 293 (5527): 111-5.
2. Chimal-Monroy, J. et al., 2003, Dev Biol  257 (2): 292-301.
3. Nakayama, N. et al., 2004, Development. 131 (1): 229-40.