Anti-Cathepsin L Antibody

Cat: 50015-RP02
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Anti-Cathepsin L Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cathepsin L Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Cathepsin L
Immunogen
Recombinant Mouse Cathepsin L / CTSL protein (Catalog#50015-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Cathepsin L / CTSL (rM Cathepsin L / CTSL; Catalog#50015-M08H; P06797; Met 1-Asn 334). Cathepsin L / CTSL specific IgG was purified by Mouse Cathepsin L / CTSL affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cathepsin L Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cathepsin L Antibody (Rabbit Polyclonal antibody) Images
Mouse Cathepsin L/CTSL1 Immunoprecipitation(IP) 8215

Mouse CTSL was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

2 µL anti-Mouse CTSL rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CTSL rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 38 kDa

Observed band size: 38 kDa

Mouse Cathepsin L/CTSL1 Western blot (WB) 13632

Anti-CTSL rabbit polyclonal antibody at 1:500 dilution

Lane A: U87MG Whole Cell Lysate

Lane B: NIH3T3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:38 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cathepsin L Antibody Alternative Names
Anti-1190035F06Rik Antibody;Anti-Ctsl1 Antibody;Anti-fs Antibody;Anti-MEP Antibody;Anti-nkt Antibody
Cathepsin L Background Information

Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. It is secreted by liver flukes at all stages of their development in the mammalian host, are believed to play important roles in facilitating parasite migration (tissue degradation), feeding and immuno-evasion. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a diagnostic or prognostic marker of human tumor malignancy.

Full Name
cathepsin L
References
  • Mulcahy G, et al. (2001) Cathepsin L proteinases as vaccines against infection with Fasciola hepatica (liver fluke) in ruminants. Res Vet Sci. 70(1): 83-6.
  • Dixit AK, et al. (2008) Immunodiagnostic/protective role of cathepsin L cysteine proteinases secreted by Fasciola species. Vet Parasitol. 154(3-4): 177-84.
  • Leto G, et al. (2010) Cathepsin L in metastatic bone disease: therapeutic implications. Biol Chem. 391(6): 655-64.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cathepsin L Antibody Rabbit Polyclonal ELISA 50015-RP01
    Anti-Cathepsin L Antibody Rabbit Monoclonal WB 10486-R221
    Anti-Cathepsin L Antibody Rabbit Monoclonal ELISA 10486-R002
    Anti-Cathepsin L Antibody Rabbit Polyclonal ELISA 10486-RP01
    Anti-Cathepsin L Antibody Rabbit Polyclonal WB,ELISA 10486-RP02
    Anti-Cathepsin L Antibody Mouse Monoclonal ELISA 10486-MM07
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