Cathepsin D / CTSD Protein (His Tag)

Cathepsin D Protein Datasheet

Cathepsin D / CTSD Protein Product Information

• Synonym: CLN10, CPSD, MGC2311
• Protein Construction: A DNA sequence encoding the pro form of human CTSD (P07339) (Met 1- Leu 412) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

Cathepsin D / CTSD Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 19
• Molecular Mass: The secreted recombinant human CTSD (pro form) consists of 405 amino acids and has a predicted molecular mass of 44 kDa. As a result of different glycosylation, the apparent molecular mass of rhCTSD is approximately 40-110 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 25mMMES, 150mMNaCl, pH6.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Cathepsin D / CTSD protein

Cathepsin D / CTSD Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cathepsin D / CTSD Protein Description

Cathepsin D (CTSD) is a well known lysosomal aspartyl protease and is a member of the peptidase C1 family found in almost all cells and tissues of mammals. It has a specificity similar to but narrower than that of pepsin A. Cathepsin D/CTSD plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Cathepsin D/CTSD is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis, and can also induce apoptosis in presence of cytotoxic factors. However, on contrast, in certain studies an inhibitory role of CTSD in apoptosis was also reviewed. In addition, Cathepsin D/CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.

References

1. Tsukuba, T. et al., 2000, Mol Cells. 10 (6): 601-611.
2. Wolf , M. et al., 2003, Am. J. Pathol. 162 (4): 1183-1190.
3. Minarowska, A. et al., 2007, Folia Histochem Cytobiol. 45 (3): 159-163.