|IP||1-2 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Mouse CTSD was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate0.5 µL anti-Mouse CTSD rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-Mouse CTSD rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 45 kDa
Observed band size: 45 kDa
Anti-CTSD rabbit monoclonal antibody at 1:500 dilution
Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:45 kDa
Observed band size:45 kDa
(We are unsure as to the identity of these extra bands.)
Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Cathepsin D Antibody||Rabbit||Polyclonal||ELISA||50127-RP01|
|Anti-Cathepsin D Antibody||Rabbit||Polyclonal||WB,ELISA,IP||50127-RP02|
|Anti-Cathepsin D Antibody||Rabbit||Monoclonal||ELISA||12517-R005|
|Anti-Cathepsin D Antibody||Rabbit||Polyclonal||WB,ELISA,IP||12517-RP02|
|Anti-Cathepsin D Antibody||Rabbit||Polyclonal||WB,ELISA||12517-RP01|
|Anti-Cathepsin D Antibody||Rabbit||Monoclonal||WB,IHC-P||12517-R010|
|Anti-Cathepsin D Antibody||Rabbit||Monoclonal||ELISA||12517-R003|