Anti-Cathepsin D Antibody

Cat: 50127-R102
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All Cathepsin D Reagents
Anti-Cathepsin D Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Cathepsin D Antibody
Validated applications
Species reactivity
Reacts with: Mouse
Mouse Cathepsin D
Recombinant Mouse Cathepsin D / CTSD Protein (Catalog#50127-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Cathepsin D / CTSD (rM Cathepsin D / CTSD; Catalog#50127-M08H; NP_034113.1; Met 1-Leu 410).
Monoclonal Rabbit IgG Clone #102
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cathepsin D Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cathepsin D Antibody (Rabbit Monoclonal antibody) Images
Mouse Cathepsin D/CTSD Immunoprecipitation(IP) 6213

Mouse CTSD was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-Mouse CTSD rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CTSD rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size: 45 kDa

Mouse Cathepsin D/CTSD Western blot (WB) 7025

Anti-CTSD rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:45 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cathepsin D Antibody Alternative Names
Anti-CatD Antibody;Anti-CD Antibody
Cathepsin D Background Information

Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.

Full Name
cathepsin D
  • Fusek M, et al. (2005) Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 149(1): 43-50.
  • Minarowska A, et al. (2007) Regulatory role of cathepsin D in apoptosis. Folia Histochem Cytobiol. 45(3): 159-63.
  • Zaidi N, et al. (2008) Cathepsin D: a cellular roadmap. Biochem Biophys Res Commun. 376(1): 5-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cathepsin D Antibody Rabbit Polyclonal ELISA 50127-RP01
    Anti-Cathepsin D Antibody Rabbit Polyclonal WB,ELISA,IP 50127-RP02
    Anti-Cathepsin D Antibody Rabbit Monoclonal ELISA 12517-R005
    Anti-Cathepsin D Antibody Rabbit Polyclonal WB,ELISA,IP 12517-RP02
    Anti-Cathepsin D Antibody Rabbit Polyclonal WB,ELISA 12517-RP01
    Anti-Cathepsin D Antibody Rabbit Monoclonal WB,IHC-P 12517-R010
    Anti-Cathepsin D Antibody Rabbit Monoclonal ELISA 12517-R003
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