Anti-Cathepsin D Antibody

Cat: 50127-RP02
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Anti-Cathepsin D Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cathepsin D Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Cathepsin D
Immunogen
Recombinant Mouse CTSD protein (Catalog#50127-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CTSD (rM CTSD; Catalog#50127-M08H; NP_034113.1; Met 1-Leu 410). CTSD specific IgG was purified by mouse CTSD affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cathepsin D Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μg/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cathepsin D Antibody (Rabbit Polyclonal antibody) Images
Mouse Cathepsin D/CTSD Western blot (WB) 13647

Anti-CTSD rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:44 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cathepsin D Antibody Alternative Names
Anti-CatD Antibody;Anti-CD Antibody
Cathepsin D Background Information

Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.

Full Name
cathepsin D
References
  • Fusek M, et al. (2005) Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 149(1): 43-50.
  • Minarowska A, et al. (2007) Regulatory role of cathepsin D in apoptosis. Folia Histochem Cytobiol. 45(3): 159-63.
  • Zaidi N, et al. (2008) Cathepsin D: a cellular roadmap. Biochem Biophys Res Commun. 376(1): 5-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cathepsin D Antibody Rabbit Monoclonal WB,ELISA,IP 50127-R102
    Anti-Cathepsin D Antibody Rabbit Polyclonal ELISA 50127-RP01
    Anti-Cathepsin D Antibody Rabbit Monoclonal ELISA 12517-R005
    Anti-Cathepsin D Antibody Rabbit Polyclonal WB,ELISA,IP 12517-RP02
    Anti-Cathepsin D Antibody Rabbit Polyclonal WB,ELISA 12517-RP01
    Anti-Cathepsin D Antibody Rabbit Monoclonal ELISA 12517-R003
    Anti-Cathepsin D Antibody Rabbit Monoclonal WB,IHC-P 12517-R010
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