Anti-Cathepsin B Antibody

Cat: 10483-RP02
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Anti-Cathepsin B Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Cathepsin B Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human Cathepsin B
Immunogen
Recombinant Human Cathepsin B / CTSB protein (Catalog#10483-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Cathepsin B / CTSB (rh Cathepsin B / CTSB; Catalog#10483-H08H; NP_001899.1). Cathepsin B / CTSB specific IgG was purified by Human Cathepsin B / CTSB affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cathepsin B Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:1000-1:4000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cathepsin B Antibody (Rabbit Polyclonal antibody) Images
Human Cathepsin B/CTSB Immunohistochemistry(IHC) 4326
Immunochemical staining of human CTSB in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human Cathepsin B/CTSB Immunohistochemistry(IHC) 4328
Immunochemical staining of human CTSB in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human Cathepsin B/CTSB Western blot (WB) 13237

Anti-CTSB rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:38 kDa

Observed band size:33 kDa

(We are unsure as to the identity of these extra bands.)

Human Cathepsin B/CTSB Immunoprecipitation(IP) 17675

CTSB was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Raw264.7 Whole Cell Lysate

Lane C:0.5 mg A549 Whole Cell Lysate

2 µL anti-CTSB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CTSB rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 38 kDa

Observed band size: 38 kDa

Anti-Cathepsin B Antibody Alternative Names
Anti-APPS Antibody;Anti-CPSB Antibody
Cathepsin B Background Information

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Full Name
cathepsin B
References
  • Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.
  • Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.
  • Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.
  • Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.
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