Cathepsin B Antibody (Antigen Affinity Purified)

Cathepsin B / CTSB Antibody (Antigen Affinity Purified) Datasheet

Cathepsin B / CTSB Antibody Product Information

• Immunogen: Recombinant Human Cathepsin B / CTSB protein (Catalog #10483-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human Cathepsin B / CTSB (rh Cathepsin B / CTSB; Catalog #10483-H08H; NP_001899.1). Cathepsin B / CTSB specific IgG was purified by Human Cathepsin B / CTSB affinity chromatography.

Cathepsin B / CTSB Antibody Usage Guide

• Specificity: Human Cathepsin B / CTSB
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CTSB in WB. Using a DAB detection system, the detection limit for Human CTSB is approximately 16 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CTSB. The detection limit for Human CTSB is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cathepsin B / CTSB Antibody Background

Human Cathepsin B (CTSB) is a papain-family cysteine protease that is normally located in lysosomes, and is a member of a superfamily of structurally similar tissue proteinases having a 25 kDa catalytic unit. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Overexpression and altered trafficking of cathepsin B characterize the malignant phenotype of tumor cells, and thus CTSB is suggested as a tumor marker.

References

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