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Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-Flag tag

  • Human Caspase-7 transcript variant alpha Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged
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Human CASP7 cDNA Clone Product Information
NCBI RefSeq:NM_001227.3
RefSeq ORF Size:912bp
cDNA Description:Full length Clone DNA of Homo sapiens caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant alpha with Flag tag.
Gene Synonym:CASP7, MCH3, CMH-1, ICE-LAP3
Species:Human
Vector:pCMV/hygro-FLAG
Plasmid:pCMV-CASP7-flag
Restriction Site:KpnI + XhoI (5.5kb + 0.94kb)
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with CASP7 qPCR primers for gene expression analysis, HP100139 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human CASP7 Gene Plasmid Map
Human Caspase-7 transcript variant alpha Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged
pCMV/hygro-FLAG Vector Information
 
Vector Name pCMV/hygro-FLAG
Vector Size 5681bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-Flag tag on other vectors
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10049-ACG$225
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10049-ACR$225
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10049-ANG$225
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10049-ANR$225
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-Flag tagHG10049-CF$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-His tagHG10049-CH$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-Myc tagHG10049-CM$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, C-HA tagHG10049-CY$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone in cloning vectorHG10049-M$75
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-Flag tagHG10049-NF$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-His tagHG10049-NH$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-Myc tagHG10049-NM$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmid, N-HA tagHG10049-NY$195
Human Caspase-7/MCH3 transcript variant alpha Gene ORF cDNA clone expression plasmidHG10049-UT$195
 Learn more about expression Vectors
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Background

Caspase 7, also known as caspase-7 and MCH3, belongs to the cysteine-aspartic acid protease (caspase) family. Caspases play a role in the signal transduction pathways of apoptosis, necrosis and inflammation. There are two major classes of caspases: initiators and effectors. The initiator isoforms (caspases-1,-4,-5,-8,-9,-10,-11,-12) are activated by, and interact with, upstream adaptor molecules through protein-protein interaction domains known as CARD and DED. Effector caspases (-3,-6,-7) are responsible for cleaving downstream substrates and are sometimes referred to as the executioner caspases. Caspase 7 exists in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. Caspase 7 cannot be detected in the brain. Caspase 7 functions in the activation cascade of caspases responsible for apoptosis execution. It cleaves and activates sterol regulatory element binding proteins (SREBPs). It proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Overexpression promotes programmed cell death.

References
  • Riedl S J, et al. (2001) Structural basis for the inhibition of caspase-3 by XIAP. Cell. 104(5):791-800.
  • Roy N, et al. (1997) The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases. EMBO J. 16(23):6914-25.
  • Deveraux Q L, et al. (1997) X-linked IAP is a direct inhibitor of cell-death proteases. Nature. 388(6639): 300-4.
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"