Caspase-14 / CASP14 Antibody

Caspase-14 / CASP14 Antibody Datasheet

Caspase-14 / CASP14 Antibody Product Information

• Immunogen: Recombinant Human Caspase-14 / CASP14 protein (Catalog#11856-H07E)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 1G6A2
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Caspase-14 / CASP14 (rh Caspase-14 / CASP14; Catalog#11856-H07E; NP_036246.1; Ser 2-Gln 242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Caspase-14 / CASP14 Antibody Usage Guide

• Specificity: Human Caspase-14 / CASP14
• No cross-reactivity in ELISA with
  E.coli cell lysate
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CASP14 in WB. Using a DAB detection system, the detection limit for Human CASP14 is approximately 20 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CASP14. The detection limit for Human CASP14 is approximately 0.16 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Caspase-14 / CASP14 Antibody Background

Caspase-14, also known as CASP-14, is a cytoplasm and nucleus protein which belongs to thepeptidase C14A family. Caspase-14 / CASP-14 is expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). It is also expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin, It is only expressed at very low levels. Caspase-14 is cleaved into the following two chains: Caspase-14 subunit p19 and Caspase-14 subunit p10. Caspase-14 / CASP-14 is believed to be a non-apoptotic caspase which is involved in epidermal differentiation. It seems to play a role in keratinocyte differentiation and cornification. Caspase-14 / CASP-14 probably regulates maturation of the epidermis by proteolytically processing filaggrin. The expression and processing of Caspase-14 may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. The CASP14 gene encodes a member of the cysteine-aspartic acidprotease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase-14 has been shown to be processed and activated by Caspase-8 and Caspase-10, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand.

References

1. Lippens S., et al., 2000, Cell Death Differ. 7: 1218-24.
2. Chien A.J., et al., 2002, Biochem. Biophys. Res. Commun. 296: 911-7.
3. Pistritto G., et al., 2002, Cell Death Differ. 9: 995-1006.
4. Demerjian,M. et al., 2008, Am J Pathol. 172 (1): 86-97.