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Caspase-14 / CASP14 Antibody, Rabbit MAb

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Human CASP14 Antibody Product Information
Immunogen:Recombinant Human Caspase-14 / CASP14 protein (Catalog#11856-H07E)
Clone ID:145
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human Caspase-14 / CASP14 (rh Caspase-14 / CASP14; Catalog#11856-H07E; NP_036246.1; Ser 2-Gln 242).
Human CASP14 Antibody Usage Guide
Specificity:Human Caspase-14 / CASP14
Application:ELISA, FCM

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CASP14. The detection limit for Human CASP14 is approximately 0. 0049 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CASP14 Antibody FC Application Image
Caspase-14 / CASP14 Antibody, Rabbit MAb, Flow cytometric
[Click to enlarge image]
Caption:
Human CASP14 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Rabbit anti-CASP14 (11856-R145, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other CASP14 Antibody Products
Reactivity: Human  
Application: ELISA  
    11856-T16-50
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      11856-MM01-50
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      Reactivity: Human  
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      11856-RP02-50
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      Application: ELISA  FCM  IF  ICC/IF  
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      11856-MM05-50
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      Reactivity: Human  
      Application: IHC-P  
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      11856-R001-50
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      Reactivity: Human  
      Application: IHC-P  IF  ICC/IF  
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      11856-R101-50
      11856-R101-100
      50 µg 
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      Reactivity: Human  
      Application: FCM  
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      11856-MM05-F-25
      11856-MM05-F-100
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      Reactivity: Human  
      Application: IHC-P  
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      11856-MM09-50
      11856-MM09-200
      11856-MM09-100
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      Reactivity: Human  
      Application: ELISA  
        11856-RP01-400
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        Caspase-14/CASP14 Background

        Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.

        Human Caspase-14/CASP14 References
      • Hu SM, et al. (1998) Caspase-14 is a novel developmentally regulated protease. The journal of biological chemistry. 273: 29648-53.
      • Marc Van De Craen, et al. (1998) Identification of a new caspase homologue: caspase-14. Cell death differ. 5(10): 838-46.
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        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"