Carboxypeptidase B2

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Carboxypeptidase B2 Related Areas

Enzyme>>Protease & Regulator>>Carboxypeptidase & Regulator>>Carboxypeptidase B2/CPB2

Carboxypeptidase B2 Alternative Names

Carboxypeptidase B2, CPB2, CPU, PCPB, TAFI [Homo sapiens]

Carboxypeptidase B2, Cpb2, 1110032P04Rik, 4930405E17Rik, AI255929, CPR, Cpu, MGC107573, TAFI [Mus musculus]

Summaries for Carboxypeptidase B2

Entrez Gene summary for Carboxypeptidase B2:

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region

OMIM - description for Carboxypeptidase B2:

Plasma carboxypeptidase B2 (CPB2; EC 3.4.17.20), also known as carboxypeptidase U (CPU) and thrombin-activatable fibrinolysis inhibitor (TAFI), is synthesized by the liver and circulates in plasma as a plasminogen-bound zymogen. When it is activated by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase B (CPB1; 114852)-like activity. CPB2 potently attenuates fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen (PLG; 173350) (summary by Zhao et al., 1998 and Boffa et al., 1999).

Wikipedia summary for Carboxypeptidase B2:

Carboxypeptidase B2 (CPB2) also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB), or thrombin-activatable fibrinolysis inhibitor (TAFI) is an enzyme that in humans is encoded by the CPB2 gene

Human Carboxypeptidase B2 Protein General Information

 

• Protein names: Recommended name: Carboxypeptidase B2
• Sequence length: 423 AA.
• Domain: Signal
• Sequence similarities: Belongs to the peptidase M14 family.
• Post-translational modification: N-glycosylated. N-glycan at Asn-108: Hex5HexNAc4.
• Enzyme regulation: TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-324.
• Subcellular location: Secreted.
• Tissue specificity: Plasma; synthesized in the liver.

General information above from UniProt

Function for Carboxypeptidase B2 Protein

UniProtKB:

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Genatlas:

• Carboxypeptidase B2 cleave C terminal basic residues
• Carboxypeptidase B2 plays a role in the protection of the clot against fibrinolysis
• Carboxypeptidase B2 exerts an antifibrinolytic effect by removing C-terminal lysines from partially degraded fibrin
• metallocarboxypeptidase (MCP) that links blood coagulation and fibrinolysis
• Carboxypeptidase B2 circulates CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses

Homology for human Carboxypeptidase B2

• homolog to murine Cpb2

Drugs for Carboxypeptidase B2

Target	Drug Name	Disease	Drug Status
Carboxypeptidase B2	AZD-9684	Thrombosis Discontinued in	Phase II

Drugs for Carboxypeptidase B2 from TTD (Therapeutic Targets Database)