|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CPM rabbit monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:51 kDa
Observed band size:55 kDa
(We are unsure as to the identity of these extra bands.)
CPM was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate2 µL anti-CPM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CPM rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 55 kDa
Observed band size: 55 kDa
Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Carboxypeptidase M Antibody||Rabbit||Monoclonal||ELISA||50990-R085|
|Anti-Carboxypeptidase M Antibody||Rabbit||Polyclonal||ELISA||50990-RP01|
|Anti-Carboxypeptidase M Antibody||Rabbit||Polyclonal||ELISA||50990-T16|
|Anti-Carboxypeptidase M Antibody||Rabbit||Polyclonal||ELISA,IHC-P||11228-T24|
|Anti-Carboxypeptidase M Antibody||Rabbit||Polyclonal||ELISA||11228-RP01|
|Anti-Carboxypeptidase M Antibody||Rabbit||Polyclonal||ELISA||11228-RP02|