Anti-Carboxypeptidase M Antibody

Cat: 11228-R105
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Anti-Carboxypeptidase M Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Carboxypeptidase M Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Carboxypeptidase M
Immunogen
Recombinant Human Carboxypeptidase M / CPM Protein (Catalog#11228-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M / CPM (rh Carboxypeptidase M / CPM; Catalog#11228-H08H; NP_938079.1; Met 1-His 422).
Source
Monoclonal Rabbit IgG Clone #105
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carboxypeptidase M Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carboxypeptidase M Antibody (Rabbit Monoclonal antibody) Images
Human Carboxypeptidase M/CPM Western blot (WB) 14779

Anti-CPM rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:51 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

Human Carboxypeptidase M/CPM Immunoprecipitation(IP) 14817

CPM was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-CPM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CPM rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 55 kDa

Carboxypeptidase M Background Information

Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.

Full Name
carboxypeptidase M
References
  • Deddish PA. et al., 1990, J Biol Chem. 265 (25): 15083-9.
  • Nagae A. et al., 1992, J Neurochem. 59 (6): 2201-12.
  • Skidgel RA. et al., 1996, Immunopharmacology. 32 (1-3): 48-52.
  • Deiteren K. et al., 2009, Clin Chim Acta. 399 (1-2): 24-39.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carboxypeptidase M Antibody Rabbit Monoclonal ELISA 50990-R085
    Anti-Carboxypeptidase M Antibody Rabbit Polyclonal ELISA 50990-RP01
    Anti-Carboxypeptidase M Antibody Rabbit Polyclonal ELISA 50990-T16
    Anti-Carboxypeptidase M Antibody Rabbit Polyclonal ELISA,IHC-P 11228-T24
    Anti-Carboxypeptidase M Antibody Rabbit Polyclonal ELISA 11228-RP01
    Anti-Carboxypeptidase M Antibody Rabbit Polyclonal ELISA 11228-RP02
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